Partial nucleotide sequence of Japanese encephalitis virus ling strain genome and comparison of the encoded structural proteins and nonstructural protein NS1 among Japanese encephalitis virus strains.

L R Jan, K L Chen, C F Lu, C B Horng
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Abstract

Approximately 4000 nucleotides of the 5'-terminal portion of Japanese encephalitis virus (JEV) Ling strain genome were cloned and sequenced. This nucleotide sequence and its encoded C-PrM-E-NS1 polyprotein sequences were also compared with the corresponding sequences of other JE virus strains. Results demonstrated that the nucleotide sequence homology varied from 97.1 to 99.3% and the amino acid homology 98.6 to 98.9%. Based on homology, the Ling strain was closer to the Beijing-1 strain than to the SA14 and JaOArS982 strains. However, only on comparison of the E sequence, which neutralization, hemagglutination-inhibition and complement fixation antigenic determinants are located, between Ling and other JEV strains demonstrated that nucleotide sequence homology varied from 97.1% to 99.3% and amino acid homology from 98.6% to 99.2%. The Ling strain JEV is more closely related to the Beijing-1 strain than to the Nakayama NIH, SA14 and JaOArS982 strains in that order. Based on this analysis, the Taiwanese JEV strain appears to be more closely related to the Chinese strain than to the Japanese strain. Also, JEV strains isolated in humans are more closely related to each other than to JEV strains of mosquito isolates.

乙型脑炎病毒ling株基因组部分核苷酸序列及编码结构蛋白与非结构蛋白NS1的比较
对日本脑炎病毒(JEV) Ling株基因组5′端约4000个核苷酸进行了克隆和测序。并将该核苷酸序列及其编码的C-PrM-E-NS1多蛋白序列与其他乙脑病毒株的相应序列进行了比较。结果表明,两种基因的核苷酸序列同源性为97.1 ~ 99.3%,氨基酸同源性为98.6 ~ 98.9%。同源性分析表明,Ling毒株与北京-1毒株的同源性比SA14和jaaoars982毒株更接近。然而,仅比较Ling株与其他乙脑毒株之间具有中和、血凝抑制和补体固定抗原决定因子的E序列,发现其核苷酸序列同源性在97.1% ~ 99.3%之间,氨基酸同源性在98.6% ~ 99.2%之间。Ling株与北京-1株的亲缘关系较好,与中山NIH株、SA14株和jaaoars982株的亲缘关系较差。基于上述分析,台湾乙脑病毒株与中国乙脑病毒株的亲缘关系比与日本乙脑病毒株的亲缘关系更密切。此外,人类分离的乙脑病毒毒株之间的亲缘关系比与蚊子分离的乙脑病毒毒株之间的亲缘关系更密切。
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