Bcl-2 Family Expression in Salivary Glands from Patients with Primary Sjögren's Syndrome: Involvement of Bax in Salivary Gland Destruction

Clinical immunology and immunopathology Pub Date : 1998-08-01 DOI:10.1006/clin.1998.4556

Liping Kong , Noriyoshi Ogawa , H.Stan McGuff , Toru Nakabayashi , Ken-mei Sakata , Reiji Masago , Norma Vela-Roch , Norman Talal , Howard Dang

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引用次数: 77

Abstract

To investigate the molecular mechanism of glandular parenchyma destruction in Sjögren's syndrome (SS), Bcl-2, Bax, Bcl-X, and Bak expression were studied. SS (n= 18) and control salivary glands (n= 6) were examined by immunohistochemistry. Apoptosis was assessed byin situDNA nick end labeling. Infiltrating mononuclear cells in the SS salivary gland showed elevated Bcl-2. These mononuclear cells expressed increased Bax but did not undergo apoptosis. Both SS and control salivary gland ductal epithelial cells expressed Bcl-2, Bax, and Bcl-X. SS, but not normal, salivary gland acinar cells expressed Bax and underwent apoptosis. These results suggest that elevated Bax expression in SS salivary gland acinar cells may play an important role in the apoptotic pathway. In contrast, Bcl-2 expression in SS infiltrating mononuclear cells and ductal cells may contribute to their survival.

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原发性Sjögren综合征患者唾液腺Bcl-2家族表达:Bax参与唾液腺破坏

为探讨Sjögren综合征(SS)中腺实质破坏的分子机制，研究了Bcl-2、Bax、Bcl-X和Bak的表达。采用免疫组化方法检测SS (n= 18)和对照组唾液腺(n= 6)。通过situDNA缺口末端标记评估细胞凋亡。SS涎腺浸润的单核细胞显示Bcl-2升高。这些单核细胞表达Bax增加，但未发生凋亡。SS和对照唾液腺导管上皮细胞均表达Bcl-2、Bax和Bcl-X。而非正常SS，唾液腺腺泡细胞表达Bax并发生凋亡。这些结果表明，Bax在SS唾液腺腺泡细胞中的表达升高可能在凋亡通路中起重要作用。相比之下，Bcl-2在浸润的单核细胞和导管细胞中的表达可能有助于它们的存活。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Clinical immunology and immunopathology

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