Immunohistochemical comparison of localization of pituitary adenylate cyclase activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP) in the enteric nerve plexus of the guinea pig jejunum.

Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity Pub Date : 1996-01-01

M Suzuki, K Nokihara, E Ando, S Naruse

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Abstract

The localization and co-localization of pituitary adenylate cyclase activating polypeptides (PACAPs) and vasoactive intestinal polypeptide (VIP) in the enteric nerve plexus of the guinea pig jejunum were immunohistochemically compared by the peroxidase anti-peroxidase (PAP) method using region-specific antisera against PACAP38, PACAP27 and VIP, respectively. Immunoreactive nerve elements were demonstrated in the ganglia of both myenteric and submucous plexi in the guinea pig jejunum. Numerous immunoreactive nerve cell bodies were localized in the myenteric ganglia, while nerve cell bodies in the submucous ganglia were only slightly immunopositive. Immunoreactive nerve terminal varicosities occurred in both myenteric and submucous ganglia. Additionally, PACAPs-immunoreactive nerve cell bodies were also immunopositive with VIP. Thus, both PACAP38 and PACAP27, as well as VIP, are co-localized in the cell bodies of guinea pig jejunum.

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豚鼠空肠肠神经丛垂体腺苷酸环化酶激活多肽(PACAP)和血管活性肠多肽(VIP)定位的免疫组化比较。

采用针对PACAP38、PACAP27和VIP的区域特异性抗血清，采用过氧化物酶抗过氧化物酶(PAP)免疫组化方法，比较垂体腺苷酸环化酶激活多肽(PACAPs)和血管活性肠多肽(VIP)在豚鼠空肠肠神经丛中的定位和共定位。在豚鼠空肠肌肠丛和粘膜下丛神经节中均可见免疫反应性神经元件。肌肠神经节内有大量的免疫反应性神经细胞体，而粘膜下神经节的神经细胞体只有轻微的免疫阳性。免疫反应性神经末梢静脉曲张发生于肌肠节和粘膜下神经节。此外，pacaps免疫反应性神经细胞体VIP免疫阳性。因此，PACAP38和PACAP27以及VIP在豚鼠空肠细胞体中共定位。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity

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