Characterization of microsomal GST-II by Western blot and identification of a novel LTC4 isomer

Abstract

Protein expression of microsomal GST-II and LTC₄ synthase was analyzed by Western blot. Correlation between a 17 kDa band and LTC₄ formation was observed for both enzymes. The expression of microsomal GST-II was several fold more efficient than the expression of LTC₄ synthase. In addition to catalyzing the biosynthesis of LTC₄, microsomal GST-II also produces another product, which has been subjected to mass spectrometric analysis. This analysis demonstrates that the novel product is an isomer of LTC₄.