Identification of a 105 kilodalton Cryptococcus neoformans mannoprotein involved in human cell-mediated immune response.

Abstract

In the present study, using sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blot techniques, the patterns of proteins extracted from C. neoformans yeast cells were analysed. A major 105 kilodalton (kDa) antigen that binds to Concanavalin A and cross-reacts with anti-mannan antibodies was identified. The 105 kDa mannoprotein, highly expressed in the acapsular mutant of C. neoformans with respect to the encapsulated strain, is involved in the lymphoproliferative response of T lymphocytes to Cryptococcus-sensitized monocytes.