{"title":"beta-endorphin1-31 in the rat pituitary.","authors":"O O Grigoriants, D M Desiderio","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Mass spectrometry was used to determine the molecular mass of rat pituitary beta-endorphin1-31 (BErat, 1-31). The measured molecular mass (3435 +/- 1 Da, n = 5) of endogenous BErat, 1-31 differed from the molecular mass of commercially available synthetic BErat, 1-31 (3465 +/- 1 Da, n = 9), but corresponded to the molecular mass of synthetic BEbovine, 1-31 (3436 +/- 3 Da, n = 3). Based on the combination of these ESIMS molecular mass measurements, HPLC retention time data, LSIMS measurement of the molecular mass of selected tryptic fragments, and consideration of codon sequences, we suggest that the amino-acid sequence of endogenous BErat, 1-31 differs from the DNA-deduced sequence of BErat, 1-31, and that endogenous BErat, 1-31 contains Ala instead of Val in position 26.</p>","PeriodicalId":14204,"journal":{"name":"International journal of peptide and protein research","volume":"47 1-2","pages":"123-30"},"PeriodicalIF":0.0000,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of peptide and protein research","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Mass spectrometry was used to determine the molecular mass of rat pituitary beta-endorphin1-31 (BErat, 1-31). The measured molecular mass (3435 +/- 1 Da, n = 5) of endogenous BErat, 1-31 differed from the molecular mass of commercially available synthetic BErat, 1-31 (3465 +/- 1 Da, n = 9), but corresponded to the molecular mass of synthetic BEbovine, 1-31 (3436 +/- 3 Da, n = 3). Based on the combination of these ESIMS molecular mass measurements, HPLC retention time data, LSIMS measurement of the molecular mass of selected tryptic fragments, and consideration of codon sequences, we suggest that the amino-acid sequence of endogenous BErat, 1-31 differs from the DNA-deduced sequence of BErat, 1-31, and that endogenous BErat, 1-31 contains Ala instead of Val in position 26.
质谱法测定大鼠垂体β -内啡肽1-31的分子质量(BErat, 1-31)。内源性BErat, 1-31的测量分子质量(3435 +/- 1 Da, n = 5)与市售合成BErat, 1-31 (3465 +/- 1 Da, n = 9)的分子质量不同，但与合成BEbovine, 1-31 (3436 +/- 3 Da, n = 3)的分子质量相对应。基于这些ESIMS分子质量测量，HPLC保留时间数据，LSIMS对所选色氨酸片段分子质量的测量，并考虑密码子序列，我们认为内源的BErat, 1-31的氨基酸序列与dna推断的BErat, 1-31的氨基酸序列不同，并且内源的BErat, 1-31在第26位含有Ala而不是Val。
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