Factors of Ehrlich Ascites Tumor resistance to oxidative stress.

P Hristov, V Tyurin, L Petrov
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Abstract

Ehrlich Ascites Tumor (EAT) cells, compared to liver, were less susceptible to peroxidation (measured by the TBARS method) induced by Fe2+/ascorbate, ADP/Fe2+ and H2O2/Fe2+/ascorbate. Higher thiobarbituric acid reagent products TBARS levels were found with Fe2+/ascorbate or after ultrasonication induced peroxidation in lipids obtained from Ehrlich cells compared to EAT cell homogenate with the same lipid content. This suggests that non-lipid and structurally membrane antioxidants factors are of less importance for induction by Fe2+/ascorbate than by ultrasonication. The resistance to induced lipid peroxidation (LPO) in EAT cells is associated with the lower level of lipids, the lower double-bond index, the lower level of fatty acids reacting with TBA, as well as with the relatively higher level of alpha-tocopherol per mg lipid in the EAT cells as compared to the liver.

埃利希腹水肿瘤抗氧化应激的影响因素。
与肝脏相比,Ehrlich腹水肿瘤(EAT)细胞对Fe2+/抗坏血酸、ADP/Fe2+和H2O2/Fe2+/抗坏血酸诱导的过氧化反应(通过TBARS法测量)较不敏感。与具有相同脂质含量的EAT细胞匀浆相比,使用Fe2+/抗坏血酸或超声诱导过氧化后,从埃利希细胞获得的脂质中发现更高的硫代巴比妥酸试剂产品TBARS水平。这表明非脂质和结构膜抗氧化剂因子对Fe2+/抗坏血酸诱导的重要性低于超声诱导。与肝脏相比,EAT细胞对诱导脂质过氧化(LPO)的抵抗与较低的脂质水平、较低的双键指数、与TBA反应的较低的脂肪酸水平以及相对较高的每毫克脂质α -生育酚水平有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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