The Effect of Oedema and Tissue Swelling on the Measurement of Neuroprotection; a Study using Chlormethiazole and Permanent Middle Cerebral Artery Occlusion in Rats

Neurodegeneration Pub Date : 1996-03-01 DOI:10.1006/neur.1996.0011

Simon G. Sydserff, Richard A. Green, Alan J. Cross

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引用次数: 22

Abstract

The effect of chlormethiazole on hemispheric swelling and cortical tissue water content has been investigated in a model of permanent middle cerebral artery (MCA) occlusion. Chlormethiazole (1 mmol/kg i.p.) or saline was administered 60 min after the induction of ischaemia and the animals sacrificed after 24 hours. The cross sectional area of the left hemisphere was increased by 21.8 + 1.9 % in saline treated rats, but only by 8.4 + 2.4% in chlormethiazole treated rats. However, the reduction in the absolute area of neurodegenerative damage (mm²) following chlormethiazole administration was considerably greater than the reduction in hemispheric swelling. Cortical tissue water content of ischaemic brain increased from 76.4% to 84.2% and this was attenuated to 78.8% by chlormethiazole administration. These data demonstrate that, providing damage is measured by fitting tissue slices onto prematched stereotactic maps, the decrease in oedema which accompanies a decrease in neurodegeneration does not result in erroneous estimates of neuroprotection.

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水肿和组织肿胀对神经保护测量的影响氯甲唑治疗大鼠永久性大脑中动脉闭塞的研究

在永久性大脑中动脉(MCA)闭塞模型中研究了氯甲唑对大脑半球肿胀和皮质组织含水量的影响。诱导缺血60 min后给予氯甲唑(1 mmol/kg i.p)或生理盐水，24 h后处死。生理盐水组大鼠左半球横截面积增加21.8 + 1.9%，而氯甲唑组仅增加8.4 + 2.4%。然而，服用氯甲唑后，神经退行性损伤绝对面积(mm2)的减少明显大于半球肿胀的减少。脑缺血脑组织皮层含水量由76.4%增加到84.2%，氯甲唑降低到78.8%。这些数据表明，通过将组织切片拟合到预先匹配的立体定向图上来测量损伤，水肿的减少伴随着神经变性的减少不会导致对神经保护的错误估计。

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Neurodegeneration

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