Neuronal nitric oxide synthase immunoreactivity in the respiratory tract of the frog, Rana temporaria.

The Histochemical Journal Pub Date : 1995-10-01
M E Bodegas, A C Villaro, L M Montuenga, S Moncada, V Riveros-Moreno, P Sesma
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Abstract

Physiological and histochemical studies have recently supported the notion that nitric oxide (NO) is the transduction signal responsible for the non-adrenergic, non-cholinergic relaxation of the vasculature as well as the airways of the mammalian lung. We report the presence of immunoreactivity to NO synthase (NOS) in nerve cell bodies and nerve fibres in the neural plexus of the buccal cavity and lungs of the frog, Rana temporaria, using the indirect immunocytochemical technique of avidin-biotin and the NADPH-diaphorase technique. The neural ganglia located next to the muscle layer and within the connective tissue of the buccal cavity were partially immunoreactive for NOS. In the lungs, NOS immunoreactivity occurred in nerve cell bodies, as well as in both myelinated and unmyelinated nerve fibres. Fine nerve fibres immunoreactive to NOS were observed within the muscle fibre bundles and next to the respiratory epithelium. Both the presence of NOS immunoreactivity and the positive histochemical reaction for NADPH-diaphorase in the neural plexus of amphibian respiratory tract suggests a broad evolutionary role for NO as a peripheral neurotransmitter.

临时蛙呼吸道神经元一氧化氮合酶免疫反应性。
最近的生理和组织化学研究支持这样一种观点,即一氧化氮(NO)是负责血管系统和哺乳动物肺部气道的非肾上腺素能、非胆碱能松弛的转导信号。我们使用亲和素生物素间接免疫细胞化学技术和NADPH-diaphorase技术,报道了在蛙颊腔和肺神经丛的神经细胞体和神经纤维中存在对NO合成酶(NOS)的免疫反应性。位于肌层旁和颊腔结缔组织内的神经节对NOS有部分免疫反应。在肺中,NOS免疫反应发生在神经细胞体以及有髓鞘和无髓鞘神经纤维中。肌纤维束内及呼吸上皮旁可见对NOS有免疫反应的细神经纤维。两栖动物呼吸道神经丛中一氧化氮的免疫反应和NADPH-diaphorase的阳性组织化学反应表明,一氧化氮作为一种外周神经递质在进化中具有广泛的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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