Expanded bed chromatography and radial flow column chromatography: the approaches for adsorption chromatography of biomolecules on industrial scale.

Abstract

Adsorption of proteins directly from unclarified feed-stocks, has gained importance in the recovery of biomolecules on Industrial scale. Adsorption in expanded beds and radial flow gives significantly better results compared to conventional column chromatography methods for downstream processing of variety of proteins from particulate-containing feed-stocks, such as fermentation broths and cells extracts. The simple operation of these techniques reduces the complexity of downstream processing by eliminating steps such as filtration, centrifugation and concentration. One step purification, with simple equipment, these techniques enable to recover pure protein with 95% yield in biotechnological industry. The principles, operating procedures, adsorbents and applications of these techniques are discussed.