Nonspecific adsorption and covalent coupling of heparin on polyacrylate based microbeads.

Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology Pub Date : 1993-01-01 DOI:10.3109/10731199309117356

A Denizli, M Kiremitçi, E Pişkin

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引用次数: 1

Abstract

Polyacrylate based microbeads were prepared by copolymerization of four different acrylate monomers, namely 2-hydroxyethylmethacrylate (HEMA), ethyleneglycoldimethacrylate (EGDMA), methylmethacrylate (MMA) and dimethylaminoethylmethacrylate (DMEAMA). These beads were further activated with CNBr at alkaline pH. The extend of nonspecific adsorption and covalent coupling of heparin on these beads were investigated in a batch reactors at different temperatures. The effects of initial concentrations of activation agent and heparin were also studied. Nonspecific heparin adsorption on the microbeads containing DMAEMA was significantly higher than the others. Nonspecific adsorption decreased with increasing temperature. Heparin was covalently coupled on CNBr activated microbeads. The amount of coupled heparin increased by increasing concentration of CNBr.

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肝素在聚丙烯酸酯微球上的非特异性吸附和共价偶联。

以2-羟乙基甲基丙烯酸酯(HEMA)、乙二醇二甲基丙烯酸酯(EGDMA)、甲基丙烯酸甲酯(MMA)和二甲氨基甲基丙烯酸乙酯(DMEAMA)四种不同的丙烯酸酯单体为共聚物，制备了聚丙烯酸酯基微球。在碱性条件下，用CNBr进一步活化这些微球。在不同温度下，研究了这些微球对肝素的非特异性吸附和共价偶联的程度。研究了活化剂初始浓度和肝素初始浓度的影响。非特异性肝素在含DMAEMA微球上的吸附显著高于其他微球。非特异性吸附随温度升高而降低。肝素在CNBr活化微球上共价偶联。随着CNBr浓度的增加，偶联肝素的量增加。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology

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