In vitro prolactin but not LH and FSH release is inhibited by compounds in extracts of Agnus castus: direct evidence for a dopaminergic principle by the dopamine receptor assay.

H Jarry, S Leonhardt, C Gorkow, W Wuttke
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引用次数: 120

Abstract

Women suffering from premenstrual mastodynia often respond to stimuli of prolactin (Prl) release with a hypersecretion of this hormone. Pharmacological reduction of Prl release by dopamine agonists or treatment with extracts of Agnus castus (AC) improve the clinical situation of patients with such premenstrual symptoms. Extracts of AC contain compounds which inhibit in vivo Prl release in women as well as in vitro from dispersed rat pituitary cells. It is yet unknown whether this inhibitory action of AC is only exerted on Prl release or whether release of other pituitary hormones like LH and FSH is also affected. The effects of AC on LH and FSH release were examined in vitro using rat pituitary cell cultures. To rule out that the Prl-inhibiting properties of AC are at least in part due to a cytotoxic component, pituitary cell cultures were subjected to the MTT test. To assess whether the Prl inhibitory effect of AC preparations is due to compounds acting as dopamine (DA) agonists, we used the corpus striatum membrane DA receptor binding assay. Our results demonstrate for the first time that AC extract contains an active principle that binds to the D2 receptor. Thus, it is very likely that it is this dopaminergic principle which inhibits Prl release in vitro from rat pituitary cells. Furthermore we give evidence for the specificity of action of AC on hormone release, since gonadotropin secretion remained unaffected. The findings of the present study support the therapeutical usefulness of AC extracts for treatment of premenstrual mastodynia which is associated with hypersecretion of Prl. Furthermore, the beneficial effects of AC appear to be due to the inhibition of pituitary Prl release.

在体外催乳素的释放,而不是黄体生成素和卵泡刺激素的释放是由化合物的提取物蓖麻:直接证据为多巴胺能原理的多巴胺受体测定。
患有经前乳突痛的妇女经常对催乳素(Prl)释放的刺激作出反应,分泌过多这种激素。通过多巴胺激动剂或菖蒲提取物减少Prl释放可改善此类经前症状患者的临床情况。AC提取物含有抑制体内Prl在女性和体外分散的大鼠垂体细胞释放的化合物。目前尚不清楚AC的这种抑制作用是否仅对Prl的释放起作用,还是对其他垂体激素如LH和FSH的释放也有影响。采用体外大鼠垂体细胞培养法检测AC对促黄体生成素和促卵泡刺激素释放的影响。为了排除AC的prl抑制特性至少部分是由于细胞毒性成分,垂体细胞培养进行了MTT试验。为了评估AC制剂的Prl抑制作用是否由于化合物作为多巴胺(DA)激动剂,我们使用了纹状体膜DA受体结合试验。我们的结果首次证明了AC提取物含有与D2受体结合的活性原理。因此,很可能正是这种多巴胺能原理抑制了大鼠垂体细胞体外释放Prl。此外,我们为AC对激素释放的特异性作用提供了证据,因为促性腺激素的分泌不受影响。本研究结果支持AC提取物对经前乳突痛的治疗作用,这与Prl的高分泌有关。此外,AC的有益作用似乎是由于抑制垂体Prl的释放。
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