Enhancement of anti-cancer activity of cisdiaminedichloroplatinum by the protein-bound polysaccharide of Coriolus versicolor QUEL (PS-K) in vitro.

Y Kobayashi, K Kariya, K Saigenji, K Nakamura
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引用次数: 21

Abstract

The protein-bound polysaccharide of Coriolus versicolor QUEL (PS_K) expresses superoxide dismutase (SOD) mimicking activity. Examination was made of the effects of PS-K on cancer cell lines following administration of the anti-cancer drug cisdiaminedichloroplatinum (cisplatin). Cell proliferation of each cell line was inhibited markedly by cisplatin from 0.5 to 5 micrograms/0.5 ml per well. Fifty percent of the inhibitory concentration (IC50) was 0.33 micrograms/0.5 ml per well in NRK-49F and human ovarian cancer cells, and 1.5 micrograms/0.5 ml per well in H4-II-E. PS-K 50 micrograms/0.5 ml per well prevented cytotoxicity due to cisplatin toward NRK-49F, but enhanced the cytotoxicity on H4-II-E and human ovarian cancer cells. Increase in lipid peroxide and decrease in SOD activity were observed following an IC50 dose of cisplatin. With PS-K 50 micrograms/0.5 ml per well, all the above were augmented in H4-II-E and ovarian cancer cells, but diminished in NRK-49F cell line. PS-K may have effect on cancer patients through its combining with cisplatin.

花椒多糖(PS-K)增强顺二胺二氯铂的体外抗癌活性
花斑Coriolus versicolor QUEL (PS_K)蛋白结合多糖具有超氧化物歧化酶(SOD)模拟活性。在使用抗癌药物顺铂后,检测了PS-K对癌细胞系的影响。顺铂用量为0.5 ~ 5微克/0.5毫升/孔,可明显抑制各细胞系的细胞增殖。50%的抑制浓度(IC50)在NRK-49F和人卵巢癌细胞中为0.33微克/0.5毫升/孔,在H4-II-E中为1.5微克/0.5毫升/孔。PS-K 50 μ g /0.5 ml /孔抑制顺铂对NRK-49F的细胞毒性,但增强对H4-II-E和人卵巢癌细胞的细胞毒性。顺铂IC50剂量后观察到脂质过氧化增加和SOD活性降低。当PS-K浓度为50微克/0.5毫升/孔时,上述活性均在H4-II-E和卵巢癌细胞中增强,但在NRK-49F细胞中减弱。PS-K可能通过与顺铂联用对癌症患者产生作用。
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