{"title":"Detection of Myosin Immunoanalogue in the Yeast Candida albicans","authors":"Machhour Ghazali, Marie-Helene Rodier, Brahim el Moudni, Nathalie Quellard, Jean-Louis Jacquemin","doi":"10.1006/emyc.1995.1012","DOIUrl":null,"url":null,"abstract":"<div><p>Ghazali, M., Rodier, M.-H., el Moudni, B., Quellard, N., Jacquemin, J.-L. 1995. Detection of myosin immunoanalogue in the yeast <em>Candida albicans. Experimental Mycology</em> 19: 101-110. Detection and localization of myosin immunoanalogue protein in the yeast <em>Candida albicans</em> were achieved by immunoblotting, indirect immunofluorescence assay, and immunoelectron microscopy. A polypeptide with an <em>M<sub>r</sub></em> about 110,000, from cytosolic extract and insoluble fraction in the corresponding membrane pellet, was reacted with polyclonal and monoclonal antibodies raised against vertebrate muscle myosin. This protein was located by immunofluorescence and immunoelectron microscopy in the cell cortex along the plasmalemma, in the cytoplasm, and in the septum corresponding to bud scar region situated between the yeast-mother cell and the bud.</p></div>","PeriodicalId":12110,"journal":{"name":"Experimental Mycology","volume":"19 2","pages":"Pages 101-110"},"PeriodicalIF":0.0000,"publicationDate":"1995-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/emyc.1995.1012","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental Mycology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0147597585710122","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
Abstract
Ghazali, M., Rodier, M.-H., el Moudni, B., Quellard, N., Jacquemin, J.-L. 1995. Detection of myosin immunoanalogue in the yeast Candida albicans. Experimental Mycology 19: 101-110. Detection and localization of myosin immunoanalogue protein in the yeast Candida albicans were achieved by immunoblotting, indirect immunofluorescence assay, and immunoelectron microscopy. A polypeptide with an Mr about 110,000, from cytosolic extract and insoluble fraction in the corresponding membrane pellet, was reacted with polyclonal and monoclonal antibodies raised against vertebrate muscle myosin. This protein was located by immunofluorescence and immunoelectron microscopy in the cell cortex along the plasmalemma, in the cytoplasm, and in the septum corresponding to bud scar region situated between the yeast-mother cell and the bud.
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