Histochemical and ultrastructural studies of mast cells in the intestinal mucosa and skin of the opossum Didelphis albiventris.

The Histochemical Journal Pub Date : 1994-03-01
A A Santos, C R Machado
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Abstract

The mast cells located in the intestinal mucosa of a non-eutherian mammal were studied in comparison with those in the skin of the ear. In the intestinal mucosa, the mast cells exhibited a more variable shape, larger cytoplasmic granules and greater sensitivity to fixatives regarding their staining towards Toluidine Blue. Ultrastructurally, these granules were more heterogeneous but lacked the crystalline content found in granules of skin mast cells; lipid body-like organelles were found only in the mucosa-located mast cells. Histochemically, they differed from skin mast cells by the absence of periodic acid-Schiff (PAS)-positive granules. Unlike the mucosal mast cells of the rat, they fluoresced brilliant yellow after berberine treatment, which is evidence of the presence of heparin.

负鼠肠黏膜和皮肤肥大细胞的组织化学和超微结构研究。
研究了非真动物肠道粘膜肥大细胞与耳皮肤肥大细胞的比较。在肠粘膜中,肥大细胞表现出更多变的形状,更大的细胞质颗粒和对固定剂更敏感的甲苯胺蓝染色。超微结构上,这些颗粒更不均匀,但缺乏皮肤肥大细胞颗粒中的晶体含量;脂质体样细胞器仅在粘膜肥大细胞中发现。组织化学上,它们与皮肤肥大细胞的不同之处在于缺乏周期性的酸-希夫(PAS)阳性颗粒。与大鼠的粘膜肥大细胞不同,在小檗碱处理后,它们发出亮黄色的荧光,这是肝素存在的证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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