Effects of arachidonic acid hydroperoxides on vascular and non-vascular smooth muscle

Prostaglandins and medicine Pub Date : 1981-12-01 DOI:10.1016/0161-4630(81)90043-4

David Aharony, J.Bryan Smith, Edward F. Smith III, Allan M. Lefer

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引用次数: 20

Abstract

The m-6 and w-9 hydroperoxides of arachidonic acid (AA) caused dose-dependent contraction of rabbit aortic strip (RAS) and guinea pig ileum (GPI) at concentrations between 5 and 200pM. At these concentrations, arachidonic acid had no effect in these preparations. The contractions could not be blocked by indomethacin, methysergide, phenoxybenzamine, propranolol, diphenhydramine, scopolamine, or the SRS-A antagonist FPL-55712, but were abolished by the calcium channel blocker nimodipine. In both tissues, the hydroperoxides initiated a sustained contraction. The onset of GPI contraction however, was much faster than the response of RAS to these hydroperoxides. 15-HPETE produced a more sustained contraction than 12-HPETE in both RAS and GPI.These results suggest that hydroperoxides generated from AA by the action of lipoxygenase can directly induce smooth muscle contraction and this effect is probably mediated through altering calcium fluxes in these smooth muscle preparations.

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花生四烯酸氢过氧化物对血管和非血管平滑肌的影响

花生四烯酸(AA)的m-6和w-9氢过氧化物在浓度为5 ~ 200pM时引起家兔主动脉带(RAS)和豚鼠回肠(GPI)的剂量依赖性收缩。在这些浓度下，花生四烯酸对这些制剂没有影响。吲哚美辛、甲塞吉特、苯氧苄胺、普萘洛尔、苯海拉明、东莨菪碱或SRS-A拮抗剂FPL-55712均不能阻断收缩，但钙通道阻滞剂尼莫地平可消除收缩。在两个组织中，氢过氧化物开始持续收缩。然而，GPI收缩的开始比RAS对这些氢过氧化物的反应要快得多。在RAS和GPI方面，15-HPETE比12-HPETE产生更持久的收缩。这些结果表明，AA在脂加氧酶的作用下产生的氢过氧化物可以直接诱导平滑肌收缩，这种作用可能是通过改变平滑肌制剂中的钙通量来介导的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Prostaglandins and medicine

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