Identification of hyaluronic acid as a component of human teratocarcinoma-derived cells of line PA 1.

Abstract

Ovarial teratocarcinoma-derived cells (PA 1) of human origin, which are known to be capable of differentiating in vitro, incorporate label from radioactive galactose and 2-amino-2-deoxy-D-glucose into hyaluronic acid. This macromolecule, which is prominent in many differentiating embryonic and malignant cells, was separated from sulfated glycosaminoglycans and from large-sized glycopeptides by chromatography on concanavalin A – Sepharose. It was identified by a number of chemical and enzymatic degradations as well as by several chromatographic methods.