Further studies on the characterization of cylindrin and torin, two extrinsic proteins of the erythrocyte membrane

J.R. Harris, I. Naeem
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引用次数: 16

Abstract

The subunit composition of the high molecular weight proteins cylindrin and torin from human erythrocyte ghosts has been studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis on 3 to 30% ‘Gradipore’ polyacrylamide gradient slab gels. Torin has been shown to be a multimer of a single polypeptide of approx. Mr 20 000. Cylindrin appears to contain five polypeptides, three of which predominate, in the Mr range 22 000 to 25 000. The isoelectric points (pI) of cylindrin and torin have been determined as 4.6 and 4.8, respectively. The molecular properties of cylindrin and torin are discussed in relation to the previous studies by the authors and others on these proteins.

进一步研究了红细胞膜外源蛋白圆柱蛋白和torin的特性
用十二烷基硫酸钠聚丙烯酰胺凝胶电泳在3 ~ 30%梯度梯度梯度板凝胶上研究了人红细胞鬼的高分子量蛋白柱状蛋白和鸟笼蛋白的亚基组成。Torin已被证明是一个多聚体的单一多肽的约。20000先生。圆柱蛋白似乎含有五种多肽,其中三种占主导地位,在Mr范围为22000至25000。测定了圆柱和圆柱的等电点pI分别为4.6和4.8。结合笔者和其他人对这两种蛋白的研究,讨论了它们的分子特性。
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