The use of 75-seleno-methionine labelled Trypanosoma brucei to measure parasite replication in vivo.

Tropenmedizin und Parasitologie Pub Date : 1983-09-01
J A MacAskill, P H Holmes
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Abstract

The suitability of 75-Se-labelled trypanosomes for the measurement of trypanosome replication rates in vivo was investigated. The principle used to estimate the doubling time of the circulating parasites was the decrease in specific activity of 10(-7) parasites and this was determined both for parasites contained in a whole blood sample and for parasites separated from the blood sample by DEAE-cellulose chromatography. When two stocks of T. brucei (TrEU 226, TrEU 667) were compared it was found that the parasitemic profiles of radio-labelled trypanosomes transfered into naive mice were essentially the same as those of unlabelled parasites of each stock. Furthermore it was found that in an acute infection (TrEU 226) there was a rapid and continuous fall in the specific activity implying a constant doubling time. However, in the more chronic relapsing infection (TrEU 667) a biphasic decrease in the specific activity occurred which although initially similar to that of the acute infection changed to a much slower rate of replication at the time of peak parasitemia. The results indicate that radiolabelled trypanosomes can be used to measure parasite replication rates in vivo, and may therefore be a valuable method for investigating the factors governing parasite growth in the circulation.

使用75-硒-蛋氨酸标记的布鲁氏锥虫来测量寄生虫在体内的复制。
研究了75硒标记锥虫在体内复制率测定中的适用性。用于估计循环寄生虫加倍时间的原理是10(-7)种寄生虫的比活性下降,这既适用于全血样本中含有的寄生虫,也适用于通过deae -纤维素色谱法从血液样本中分离出来的寄生虫。对两种布鲁氏锥虫种群(TrEU 226和TrEU 667)进行比较发现,放射性标记的锥虫转移到幼鼠体内的寄生虫谱与未标记的两种种群的寄生虫谱基本相同。此外,发现在急性感染(TrEU 226)中,特异性活性快速持续下降,这意味着恒定的倍增时间。然而,在更慢性的复发性感染(TrEU 667)中,特异性活性出现双期下降,尽管最初与急性感染相似,但在寄生虫血症高峰时,复制速度变慢。结果表明,放射性标记锥虫可以用来测量寄生虫在体内的复制率,因此可能是研究寄生虫在循环中生长的控制因素的有价值的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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