Effect of diethylcarbamazine on serum-dependent cell-mediated immune reactions to microfilariae in vitro.

Tropenmedizin und Parasitologie Pub Date : 1984-09-01

R Chandrashekar, U R Rao, D Subrahmanyam

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Abstract

The effect of diethylcarbamazine citrate (DEC) on serum-dependent cellular adherence and cytotoxicity to sheathed and exsheathed microfilariae of Litomosoides carinii and Brugia pahangi in vitro has been investigated. DEC at 5 micrograms/ml promoted antibody-mediated neutrophil adherence and cytotoxicity with sheathed L. carinii microfilariae. With exsheathed parasites, the drug promoted significantly complement-and antibody-mediated cellular adherence and cytotoxicity. In the case of B. pahangi, antibody-dependent cell-mediated cytotoxicity (ADCC) of neutrophils to sheathed and exsheathed microfilariae were similarly stimulated by DEC. Pre-incubation of microfilariae with DEC promoted the cellular adherence and cytotoxicity whereas pre-incubation of cells had no such effect. DEC stimulated the adherence of eosinophils to both sheathed and exsheathed microfilariae of the two species but the adherence resulted in cytotoxic effect only on the L. carinii microfilariae. DEC at higher concentrations (100 or 500 micrograms/ml) inhibits these processes. Data on the role of different serum factors involved in this phenomenon have been presented and discussed.

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二乙基卡马嗪对体外微丝虫病血清依赖细胞免疫反应的影响。

研究了枸橼酸二乙基卡马嗪(DEC)对卡氏毛线虫(Litomosoides carinii)和帕汉吉氏毛线虫(Brugia pahangi)鞘外微丝体外细胞粘附及细胞毒性的影响。5微克/毫升的DEC促进了抗体介导的中性粒细胞粘附和对有鞘的卡氏乳杆菌微丝的细胞毒性。对于脱出的寄生虫，该药物显著促进补体和抗体介导的细胞粘附和细胞毒性。在pahangi的实验中，DEC同样刺激了中性粒细胞对被鞘和被鞘微丝的抗体依赖细胞介导的细胞毒性(ADCC)，而DEC对微丝的预孵育促进了细胞粘附和细胞毒性，而细胞的预孵育则没有这种作用。DEC刺激了嗜酸性粒细胞对两种微丝的粘附，但这种粘附仅对卡氏乳杆菌微丝产生细胞毒作用。较高浓度的DEC(100或500微克/毫升)抑制这些过程。有关这一现象中涉及的不同血清因子的作用的数据已被提出和讨论。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Tropenmedizin und Parasitologie

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