[Partial purification of the extracellular proteolytic system of Aeromonas hydrophila LP50: comparative chromatographic and electrophoretic study].

Abstract

A complex extracellular proteolytic system was produced by Aeromonas hydrophila LP50 on glucose- polypeptone medium. Partial purification of this system was accomplished by ammonium sulphate precipitation, acetone precipitation, gel filtration on Sephacryl- S200 and chromatography on DEAE-Sephacel. Every stage was controlled by electrophoresis. This proteolytic system was constituted of three aminopeptidase and two endopeptidase components.