The Ca2+-sensitive K+ transport in inside-out red cell membrane vesicles.

J Szebeni
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引用次数: 0

Abstract

Ca2+-sensitive K+ transport ("Gárdos-effect") was investigated in inside-out red cell membrane vesicles (IOVs) by measuring the effect of Ca2+ and other agents on the 86Rb+ (K+-analogue) influx. 50 micro M-5 mM Ca2+ stimulated the 86Rb+ influx as compared to the effect of 1 mM EDTA. Mg2+ and chlorpromazine inhibited the effect of Ca2+, oligomycin and quinine were without effect. The permeability increase of K+, elicited by Ca2+ in IOVs, corresponded to that of intact cells. The effect of Ca2+ was not specific to K+ with respect to Na+. The Ca2+-sensitive K+ channels saturated about 5-10 mM KCl in the medium. The results indicate that IOVs the Ca2+-sensitive K+ transport has altered characteristics as compared to that of intact cells and resealed ghosts. However, in contrast to the suggestion of other investigators, it was found that some features of this phenomenon are still preserved in IOVs.

Ca2+敏感的K+在内向外的红细胞膜囊泡中的转运。
通过测量Ca2+和其他药物对86Rb+ (K+类似物)内流的影响,研究了Ca2+敏感的K+运输(“Gárdos-effect”)在内向外的红细胞膜囊泡(IOVs)中。与1 mM EDTA相比,50微M-5 mM Ca2+刺激了86Rb+内流。Mg2+和氯丙嗪抑制Ca2+的作用,低霉素和奎宁没有作用。Ca2+在IOVs中引起的K+通透性增加与完整细胞相对应。相对于Na+, Ca2+的作用不是K+所特有的。Ca2+敏感的K+通道在介质中饱和约5-10 mM KCl。结果表明,与完整细胞和重封鬼相比,IOVs的Ca2+敏感K+转运特性发生了变化。然而,与其他研究者的建议相反,发现这种现象的一些特征在IOVs中仍然保留。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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