{"title":"Isolation and characterization of tributyltin resistant mutants of Escherichia coli.","authors":"A P Singh, K Singh","doi":"10.1515/znc-1984-3-416","DOIUrl":null,"url":null,"abstract":"<p><p>Two classes of tributyltin (TBT) resistant, spontaneous mutants of Escherichia coli K-12 were isolated, using a cytochrome containing (W 1485) and a cytochrome deficient ( SASX76 ) strain. In contrast to the cytochrome sufficient strain, the cytochrome deficient strain was found to be fifty times more sensitive to TBT. The class I mutants, isolated from strain W 1485, also showed cross-resistance to triphenyltin (TPT). As compared to its wild type parent, the TBT-resistant mutants exhibited mucoid colony type, aberrant cell morphology and reduced uptake of TPT. Based on these results, it was suggested that the resistance of class I mutants to TBT may be associated with above mentioned alterations. The class II TBT-resistant mutants were isolated from the cytochrome deficient strain, SASX76 . In comparison to class I mutants, these class II mutants were found to have TBT-resistant membrane bound adenosine triphosphatase (ATPase) which may account for their resistance to TBT.</p>","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"39 3-4","pages":"293-9"},"PeriodicalIF":0.0000,"publicationDate":"1984-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-1984-3-416","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift fur Naturforschung. Section C, Biosciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/znc-1984-3-416","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
Abstract
Two classes of tributyltin (TBT) resistant, spontaneous mutants of Escherichia coli K-12 were isolated, using a cytochrome containing (W 1485) and a cytochrome deficient ( SASX76 ) strain. In contrast to the cytochrome sufficient strain, the cytochrome deficient strain was found to be fifty times more sensitive to TBT. The class I mutants, isolated from strain W 1485, also showed cross-resistance to triphenyltin (TPT). As compared to its wild type parent, the TBT-resistant mutants exhibited mucoid colony type, aberrant cell morphology and reduced uptake of TPT. Based on these results, it was suggested that the resistance of class I mutants to TBT may be associated with above mentioned alterations. The class II TBT-resistant mutants were isolated from the cytochrome deficient strain, SASX76 . In comparison to class I mutants, these class II mutants were found to have TBT-resistant membrane bound adenosine triphosphatase (ATPase) which may account for their resistance to TBT.