Monoclonal antibodies against pregnancy-specific beta 1-glycoprotein (SP1) in immunohistochemistry and radioimmunoassay.

Abstract

Monoclonal mouse antibodies against pregnancy-specific beta-1-glycoprotein (SP1) have been studied for their suitability in immunoperoxidase staining and radioimmunoassay methodologies. These antibodies were useful in staining normal placentas, hydatidiform moles, invasive moles and choriocarcinomas. They showed good specificity, with minimal background staining, and will thus be superior to conventional polyclonal antisera in immunohistochemistry. However, the presently tested monoclonal anti-SP1 antibodies were found not to be suitable for radioimmunoassay.