Surface amplification of pencil graphite electrode with polypyrrole and reduced graphene oxide for fabrication of a guanine/adenine DNA based electrochemical biosensors for determination of didanosine anticancer drug

IF 6.3 2区材料科学 Q2 CHEMISTRY, PHYSICAL

Applied Surface Science Pub Date : 2018-05-31 DOI:10.1016/j.apsusc.2018.01.237

Hassan Karimi-Maleh , Asma Bananezhad , Mohammad R. Ganjali , Parviz Norouzi , Abdolhossein Sadrnia

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引用次数: 97

Abstract

Didanosine is nucleoside analog reverse transcriptase inhibitors with many side effects such as nausea and vomiting, stomach pain, tingling, burning and numbness and determination of this drug is very important in biological samples. This paper presents a DNA biosensor for determination of didanosine (DDI) in pharmaceutical samples. A pencil graphite electrode modified with conductive materials such as polypyrrole (PPy) and reduced graphene oxide (rGO) (PGE/PPy/rGO) was used for this goal. The double-stranded DNA was successfully immobilized on PGE/PPy/rGO. The PGE/PPy/rGO was characterized by microscopic and electrochemical methods. Then, the interaction of DDI with DNA was identified by decreases in the oxidation currents of guanine and adenine by differential pulse voltammetric (DPV) method. The dynamic range of DDI identified in the range of 0.02–50.0 µM and this electrode provided a low limit of detection (LOD = 8.0 nM) for DDI. The PGE/PPy/rGO loaded with ds-DNA was utilized for the measurement of DDI in real samples and obtained data were compared with HPLC method. The statistical tests such as F-test and t-test were used for confirming ability of PGE/PPy/rGO loaded with ds-DNA for analysis of DDI in real samples.

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用聚吡咯和还原氧化石墨烯对铅笔石墨电极进行表面扩增，制备鸟嘌呤/腺嘌呤DNA电化学生物传感器，用于测定抗癌药物二腺苷

二腺苷是核苷类类似逆转录酶抑制剂，具有恶心、呕吐、胃痛、刺痛、灼烧和麻木等副作用，在生物样品中测定该药物非常重要。介绍了一种用于药物样品中二腺苷(DDI)测定的DNA生物传感器。用导电材料如聚吡咯(PPy)和还原氧化石墨烯(rGO) (PGE/PPy/rGO)修饰铅笔石墨电极来实现这一目标。双链DNA成功地固定在PGE/PPy/rGO上。通过微观和电化学方法对PGE/PPy/rGO进行了表征。然后，用差分脉冲伏安法(DPV)通过鸟嘌呤和腺嘌呤氧化电流的降低来鉴定DDI与DNA的相互作用。DDI的动态范围为0.02 ~ 50.0 µM，该电极对DDI的检出下限为 = 8.0 nM。利用负载ds-DNA的PGE/PPy/rGO测定实际样品中的DDI，并与HPLC法进行比较。采用f检验和t检验等统计检验，确认PGE/PPy/rGO装载ds-DNA用于实际样品DDI分析的能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Applied Surface Science 工程技术-材料科学：膜

CiteScore

12.50

自引率

7.50%

发文量

3393

审稿时长

67 days

期刊介绍： Applied Surface Science covers topics contributing to a better understanding of surfaces, interfaces, nanostructures and their applications. The journal is concerned with scientific research on the atomic and molecular level of material properties determined with specific surface analytical techniques and/or computational methods, as well as the processing of such structures.