Sensitivity improvements in spectrophospho-fluorimetry.

Abstract

The construction of a sensitive spectrophosphofluorimeter is reported. The sensitivity of the detection system has been increased by the use of a cooled selected photomultiplier and a lock-in amplifier or time averaging computer. Essential accessories are the electronic ratio system and the xenon arc stabilization device with a stabilization factor up to 103. Measurements of decay times greater than 0·1 ms for weak signals are possible with this instrument.