Determination of 3β-hydroxy-5-cholenoic acid in serum of hepatobiliary diseases—Its glucuronidated and sulfated conjugates

Biochemical medicine Pub Date : 1985-06-01 DOI:10.1016/0006-2944(85)90016-X

Hajime Takikawa , Hideaki Otsuka , Tomoe Beppu , Yousuke Seyama

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引用次数: 10

Abstract

3β-Hydroxy-5-cholenoic acid in the serum of control subjects and 62 patients with various hepatobiliary diseases was quantitated by mass fragmentography after separation into nonglucuronidated-nonsulfated, glucuronidated, and sulfated fractions. Deuterium-labeled deoxycholic acid and its glucuronide and sulfate were used as internal standards. Mean concentrations of total 3β-hydroxy-5-cholenoic acid in serum (μmole/liter) were as follows: Control subjects (14), 0.184; obstructive jaundice (15), 6.783; liver cirrhosis, compensated (12), 0.433, and decompensated (12), 1.636; chronic hepatitis (12), 0.241; and acute hepatitis (11), 2.364. Most of the 3β-hydroxy-5-cholenoic acid was glucuronidated or sulfated. Only in patients with obstructive jaundice did glucuronidation (60 ± 14%) exceed sulfation (31 ± 14%), sulfation exceeding glucuronidation in the others. The UDP-glucuronyltransferase might have different substrate specificities for 3β-hydroxy-5-cholenoic acid and other common bile acids, especially in the cholestatic state.

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肝胆疾病患者血清中3β-羟基-5-胆烯酸的测定及其糖醛酸和硫酸盐缀合物

将对照组和62例各种肝胆疾病患者血清中3β-羟基-5-胆烯酸分成非糖醛酸-非硫酸、糖醛酸和硫酸三部分，用质量碎片术定量测定。以氘标记的去氧胆酸及其葡萄糖醛酸盐和硫酸盐为内标。血清中总3β-羟基-5-胆烯酸平均浓度(μmol /l)如下:对照组(14例)，0.184;梗阻性黄疸(15)，6.783;肝硬化，代偿(12)，0.433，失代偿(12)，1.636;慢性肝炎(12)，0.241;急性肝炎(11)，2.364。3β-羟基-5-胆烯酸大部分被葡萄糖醛酸化或硫酸酸化。只有梗阻性黄疸患者糖醛酸化(60±14%)超过硫酸化(31±14%)，硫酸化超过糖醛酸化(31±14%)。udp -葡萄糖醛基转移酶对3β-羟基-5-胆酸和其他常见胆汁酸可能具有不同的底物特异性，特别是在胆汁淤积状态下。

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Biochemical medicine

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