Advanced optical methods and strategies for extracellular vesicles characterization and dynamic studies

IF 2.8 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chemistry and Physics of Lipids Pub Date : 2026-04-01 Epub Date: 2026-03-25 DOI:10.1016/j.chemphyslip.2026.105576

Zike Zhang , Futao Meng , Chao Wang , Qiao Mei

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引用次数: 0

Abstract

Extracellular vesicles (EV) can serve as nanocarriers for drug delivery, but their clinical translation is hampered by challenges in characterizing their dynamic interactions with target cells. Moreover, the dynamic reaction between EV and target cells remains unclear, especially at single-particle level. Recent techniques in advanced microscopy and artificial intelligence (AI) now present an opportunity to overcome this bottleneck. Therefore, the goal of this review is to evaluate how these technologies can be integrated to provide a dynamic, and label-free understanding of EV biology. In this review, we discuss the properties of the EV and commonly used probes for EV tracking. We also review the latest advances in microscopic techniques for visualizing EV uptake. Furthermore, we explore the potential of AI driven image analysis from these complex processes. By emphasizing the importance of these techniques, this review aims to provide a clear roadmap for researchers to leverage these new tools and ultimately provide new perspectives for EV-based therapeutics.

查看原文本刊更多论文

细胞外囊泡表征和动态研究的先进光学方法和策略。

细胞外囊泡（EV）可以作为药物递送的纳米载体，但由于其与靶细胞的动态相互作用特征的挑战，它们的临床转化受到阻碍。此外，EV与靶细胞之间的动态反应尚不清楚，特别是在单颗粒水平上。先进显微镜和人工智能（AI）的最新技术为克服这一瓶颈提供了机会。因此，本综述的目的是评估如何将这些技术整合起来，以提供对EV生物学动态的、无标签的理解。在本文中，我们讨论了EV的特性和常用的EV跟踪探头。我们还回顾了可视化EV摄取的显微技术的最新进展。此外，我们从这些复杂的过程中探索人工智能驱动的图像分析的潜力。通过强调这些技术的重要性，本综述旨在为研究人员提供一个清晰的路线图，以利用这些新工具，并最终为基于ev的治疗提供新的视角。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemistry and Physics of Lipids 生物-生化与分子生物学

CiteScore

7.60

自引率

2.90%

发文量

审稿时长

40 days

期刊介绍： Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.