Host-derived chitinase orchestrates gut-liver dialogue to improve growth and immunity in dark sleeper (Odontobutis potamophila)

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2026-04-15 Epub Date: 2026-02-02 DOI:10.1016/j.aqrep.2026.103418

Siyu Lu , Chuangchuang Wei , Jinjuan Wan , Yuning Hu , Yu Xu , Zhiqiang Xu , Jun Zhou , Yanfei Deng , Huimin Zhang , Xuguang Li

{"title":"Host-derived chitinase orchestrates gut-liver dialogue to improve growth and immunity in dark sleeper (Odontobutis potamophila)","authors":"Siyu Lu , Chuangchuang Wei , Jinjuan Wan , Yuning Hu , Yu Xu , Zhiqiang Xu , Jun Zhou , Yanfei Deng , Huimin Zhang , Xuguang Li","doi":"10.1016/j.aqrep.2026.103418","DOIUrl":null,"url":null,"abstract":"<div><div>Exogenous chitinase has potential to improve growth performance and health in aquatic species, but the underlying mechanisms remain incompletely understood. This study investigated the effects of graded doses of recombinant <em>Odontobutis potamophila</em> chitinase (OpCht) in <em>O. potamophila</em> fed a high-chitin diet for 8 weeks. Results demonstrated dose-dependent improvements in growth performance and hepatointestinal health. Gut metagenomics showed enrichment of orders Chitinophagales and Flavobacteriales and increased abundance of chitin-related CAZyme families (e.g., GH18 and GH19), together with functional shifts involving lipid metabolism and immune-related pathways. Liver transcriptomics revealed that medium/high-dose OpCht significantly enhanced lipid transport genes (<em>fxr</em>, <em>angptl4</em>, <em>apoa</em>) and immune effectors (<em>c3</em>, <em>il-10r</em>). Crucially, correlation analyses identified significant correlations between specific microbiota and host genes: Flavobacteriales abundance positively correlated with the hepatic lipid regulator <em>angptl4</em>, while Chitinophagales abundance correlated with the bile acid receptor <em>fxr</em>. These findings suggest that OpCht may both facilitate dietary chitin breakdown and remodel gut microbiome functionality, which is associated with enhanced chitin utilization and metabolite generation, thereby supporting a putative gut-liver axis contributing to improved host physiological functions.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"47 ","pages":"Article 103418"},"PeriodicalIF":3.7000,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture Reports","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S235251342600058X","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/2/2 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}

引用次数: 0

Abstract

Exogenous chitinase has potential to improve growth performance and health in aquatic species, but the underlying mechanisms remain incompletely understood. This study investigated the effects of graded doses of recombinant Odontobutis potamophila chitinase (OpCht) in O. potamophila fed a high-chitin diet for 8 weeks. Results demonstrated dose-dependent improvements in growth performance and hepatointestinal health. Gut metagenomics showed enrichment of orders Chitinophagales and Flavobacteriales and increased abundance of chitin-related CAZyme families (e.g., GH18 and GH19), together with functional shifts involving lipid metabolism and immune-related pathways. Liver transcriptomics revealed that medium/high-dose OpCht significantly enhanced lipid transport genes (fxr, angptl4, apoa) and immune effectors (c3, il-10r). Crucially, correlation analyses identified significant correlations between specific microbiota and host genes: Flavobacteriales abundance positively correlated with the hepatic lipid regulator angptl4, while Chitinophagales abundance correlated with the bile acid receptor fxr. These findings suggest that OpCht may both facilitate dietary chitin breakdown and remodel gut microbiome functionality, which is associated with enhanced chitin utilization and metabolite generation, thereby supporting a putative gut-liver axis contributing to improved host physiological functions.

查看原文本刊更多论文

宿主来源的几丁质酶协调肠道-肝脏对话，促进黑暗睡眠者的生长和免疫

外源性几丁质酶有可能改善水生物种的生长性能和健康，但潜在的机制仍不完全清楚。本研究研究了在高几丁质饲料喂养8周后，分级剂量重组嗜马铃薯齿蟾几丁质酶（OpCht）对嗜马铃薯齿蟾的影响。结果显示，剂量依赖性的生长性能和肝肠健康的改善。肠道宏基因组学显示，几丁质噬菌目和黄杆菌目的富集，几丁质相关CAZyme家族（如GH18和GH19）的丰度增加，以及涉及脂质代谢和免疫相关途径的功能改变。肝脏转录组学显示，中/高剂量OpCht显著增强了脂质转运基因（fxr, angptl4, apoa）和免疫效应基因（c3, il-10r）。至关重要的是，相关分析确定了特定微生物群与宿主基因之间的显著相关性：黄杆菌群丰度与肝脂质调节剂angptl4呈正相关，而几丁质噬菌群丰度与胆汁酸受体fxr相关。这些发现表明，OpCht可能既促进饮食中几丁质的分解，又重塑肠道微生物群功能，这与几丁质的利用和代谢物的产生有关，从而支持假定的肠-肝轴有助于改善宿主生理功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.