Three-in-one: A pre-tetrahedral DNA biosensor for vascular endothelial growth factor (VEGF) mRNA detection, imaging, and immobilization

IF 4.6 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2026-05-15 Epub Date: 2026-02-05 DOI:10.1016/j.saa.2026.127557

Xueqing Tao , Shengmei Wang , Ting Zhou , Aidi Tong , Wei Zou , Chunyi Tong , Kun Zhang

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引用次数: 0

Abstract

Vascular endothelial growth factor (VEGF) mRNA has garnered widespread attention as an important biomarker for disease diagnosis and treatment. However, the rapid and sensitive monitoring of its expression levels remains challenging. Herein, we have developed a pre-tetrahedral DNA (pre-TDN) biosensor for in vitro detection, in situ imaging and immobilization of VEGF mRNA. The pre-TDN biosensor was composed of a FAM-labeled pre-TDN structure and a short DABCYL-labeled DNA strand. The pre-TDN structure was self-assembled from three of the four tetrahedral DNA strand typically forming a tetrahedral DNA nanostructure. The presence of VEGF mRNA as the fourth strand completes the assembly into a tetrahedral nanostructure, leading to fluorescence emission. This biosensor has a limit of detection (LOD) of 1.01 nM. In addition, it enables intracellular specific imaging while simultaneously suppressing VEGF mRNA expression, extending therapeutic possibilities for related diseases. In conclusion, the pre-TDN biosensor for VEGF mRNA detection system provides a general analytical idea for the detection of other mRNAs, which is expected to be applied to the diagnosis and treatment of clinical diseases.

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三合一：用于血管内皮生长因子（VEGF） mRNA检测、成像和固定的四面体前DNA生物传感器

血管内皮生长因子（VEGF） mRNA作为疾病诊断和治疗的重要生物标志物受到广泛关注。然而，快速和敏感地监测其表达水平仍然具有挑战性。在此，我们开发了一种用于体外检测、原位成像和固定化VEGF mRNA的预四面体DNA （pre-TDN）生物传感器。该pre-TDN生物传感器由fam标记的pre-TDN结构和dabcyl标记的短DNA链组成。预tdn结构由四条四面体DNA链中的三条自组装而成，通常形成四面体DNA纳米结构。VEGF mRNA作为第四链的存在完成了四面体纳米结构的组装，导致荧光发射。该传感器的检测限为1.01 nM。此外，它可以在抑制VEGF mRNA表达的同时实现细胞内特异性成像，扩大相关疾病的治疗可能性。综上所述，pre-TDN生物传感器用于VEGF mRNA检测系统为其他mRNA的检测提供了一个通用的分析思路，有望应用于临床疾病的诊断和治疗。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.