Optimization of agarose-gelatin double embedding to minimize sectioning artifacts in multicellular spheroids.

IF 2.5 4区工程技术 Q3 BIOCHEMICAL RESEARCH METHODS

BioTechniques Pub Date : 2026-01-01 Epub Date: 2026-02-02 DOI:10.1080/07366205.2026.2621053

Hyoyoung Maeng, Min-Gi Han, Yoseop Jeon, Donghyeon Kim, Yuna Park, Hyuk Song

引用次数: 0

Abstract

Processing methods that do not induce shape distortion are essential for the analysis of tissue morphology. Although the use of agarose-gelatin double embedding to reduce shape distortion has been suggested, the effect of matrix concentration has not been addressed. Therefore, combinations of agarose (1-3%) and gelatin (1-10%) were evaluated using multicellular spheroids without extracellular matrix as a model of mechanical fragility. In this study, a blend of 2% agarose and 5% gelatin effectively prevented distortion. This protocol can enhance the morphological analysis of delicate tissues.

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琼脂糖-明胶双包埋工艺优化以减少多细胞球体切片伪影。

不引起形状畸变的处理方法对于组织形态分析至关重要。虽然有人建议使用琼脂糖-明胶双包埋来减少形状畸变，但基质浓度的影响尚未得到解决。因此，琼脂糖（1-3%）和明胶（1-10%）的组合使用无细胞外基质的多细胞球体作为机械脆性模型进行评估。在这项研究中，2%琼脂糖和5%明胶的混合物有效地防止了变形。这种方法可以增强对脆弱组织的形态学分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BioTechniques 工程技术-生化研究方法

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

3.3 months

期刊介绍： BioTechniques is a peer-reviewed, open-access journal dedicated to publishing original laboratory methods, related technical and software tools, and methods-oriented review articles that are of broad interest to professional life scientists, as well as to scientists from other disciplines (e.g., chemistry, physics, computer science, plant and agricultural science and climate science) interested in life science applications for their technologies. Since 1983, BioTechniques has been a leading peer-reviewed journal for methods-related research. The journal considers: Reports describing innovative new methods, platforms and software, substantive modifications to existing methods, or innovative applications of existing methods, techniques & tools to new models or scientific questions Descriptions of technical tools that facilitate the design or performance of experiments or data analysis, such as software and simple laboratory devices Surveys of technical approaches related to broad fields of research Reviews discussing advancements in techniques and methods related to broad fields of research Letters to the Editor and Expert Opinions highlighting interesting observations or cautionary tales concerning experimental design, methodology or analysis.