Monoclonal antibody against TXB2: Its use in solid and liquid phase radirmmunoassays

Abstract

Monoclonal antibodies to thromboxane B₂ (TXB₂) have been produced and characterized. Both liquid and solid phase radio immunoassays have been developed using one of these monoclonal antibodies. The two assays gave similar results when used to quantitate TXB₂ in 11 serum samples; however the solid phase assay was more sensitive than the liquid phase assay (i.e., 63 pg/ml vs 19 pg/ml) at a B/B₀ = 90%). Despite a difference in the sensitivity of the two assay systems, the cross-reactivity of the monoclonal antibody for PGD₂, PGE₂, PGF_2α and 6 keto-PGF_1α was the same.