Effects of rumen-protected or unprotected Clostridium butyricum on growth performance, rumen fermentation, immunity and antioxidation in fattening goats.

IF 2.6 3区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
PLoS ONE Pub Date : 2025-10-10 eCollection Date: 2025-01-01 DOI:10.1371/journal.pone.0330512
Caixia Zhang, Jiyu Han, Hairong Wang
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引用次数: 0

Abstract

Probiotics have been suggested to promote growth and immune performance in animals through long-term feeding. Among probiotics, Clostridium butyricum (C. butyricum) has been shown to have varying effects on the growth of ruminants. However, the impact of rumen-protected C. butyricum on ruminant organisms remains unclear. This study aimed to investigate the effects of rumen-protected and rumen-unprotected C. butyricum on the growth and plasma metabolites of fattening goats after lipopolysaccharide (LPS) challenge. A total of 24 fattening goats, aged 7-8 months, were randomly assigned to one of the following treatments: (1) control (CON); (2) unprotected C. butyricum (CB); and (3) rumen-protected C. butyricum (RPCB). After a 10-week feeding experiment (including two weeks of preparatory experiments), the goats were injected subcutaneously with LPS (1 μg/kg BW). The three treatments did not significantly affect growth performance, digestibility, or antioxidant enzyme activity (P > 0.05). However, the RPCB group showed a greater ability to reduce propionic acid (35.8%) in the rumen (P < 0.01) than did the CB group, thus alleviating the decrease in rumen pH (CB = 5.93, RPCB = 6.13, P < 0.01). Furthermore, the β-oxidation products in the plasma increased in both the CB and RPCB groups (P < 0.01). The difference in the fecal flora between the CB and RPCB groups was limited, but the content of harmful bacteria in the feces of the other two groups decreased compared with that in the CON group (P < 0.01). Unprotected C. butyricum increased the concentration of IgM after LPS injection (P < 0.01). 8-Amino-7-oxononanoate (KAPA) can serve as a biomarker for the effect of C. butyricum on the body. Overall, although rumen-protected C. butyricum could alleviate the decrease in rumen pH, our results suggest that direct feeding of C. butyricum could help improve the immune performance of fattening goats.

保护或不保护瘤胃丁酸梭菌对育肥山羊生长性能、瘤胃发酵、免疫和抗氧化的影响。
益生菌被认为可以通过长期喂养来促进动物的生长和免疫性能。在益生菌中,丁酸梭菌(C. butyricum)对反刍动物的生长有不同的影响。然而,保护瘤胃的丁酸梭菌对反刍生物的影响尚不清楚。本试验旨在研究保护瘤胃和不保护瘤胃的丁酸梭菌对脂多糖(LPS)刺激后育肥山羊生长和血浆代谢产物的影响。选取24只7 ~ 8月龄的育肥山羊,随机分为两组:(1)对照组(CON);(2)未保护丁酸梭菌(CB);(3)瘤胃保护性丁酸梭菌(RPCB)。饲喂试验10周(含预备试验2周)后,分别皮下注射LPS (1 μg/kg BW)。3种处理对生长性能、消化率和抗氧化酶活性均无显著影响(P < 0.05)。然而,RPCB组对瘤胃丙酸的还原能力更强(35.8%)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
PLoS ONE
PLoS ONE 生物-生物学
CiteScore
6.20
自引率
5.40%
发文量
14242
审稿时长
3.7 months
期刊介绍: PLOS ONE is an international, peer-reviewed, open-access, online publication. PLOS ONE welcomes reports on primary research from any scientific discipline. It provides: * Open-access—freely accessible online, authors retain copyright * Fast publication times * Peer review by expert, practicing researchers * Post-publication tools to indicate quality and impact * Community-based dialogue on articles * Worldwide media coverage
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