Ecdysone signaling-induced dumpless1 expression controls nurse cell dumping in Drosophila oogenesis.

IF 15.7 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Jingshan Li,Zishan Pan,Xiaoqi Peng,Yu Feng,Junfeng Wu,Feiying Liang,Qili Feng,XiaoQiang Yu,Huimin Deng
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引用次数: 0

Abstract

Nurse cell (NC) dumping, a process essential for oocyte development, involves the rapid cytoplasmic transfer from germline-derived NCs into the oocyte. However, its regulatory mechanism remains unclear. Here, we report that ecdysone signaling in stretch follicle cells (SFCs) regulates NC dumping through dumpless1, a ZAD-C2H2 zinc finger transcription factor, in Drosophila. Ecdysone induced dumpless1 expression in SFCs, and CRISPR/Cas9-mediated knockout of dumpless1 or its functional domain ZAD suppresses NC dumping. Depletion of dumpless1 upregulates integrin βPS expression in SFC plasma membrane, while reducing cortical enrichment of Rho1 signaling-dependent phosphorylated myosin light chain (p-MLC) and disrupting actin cables organization in NCs. SFC-specific overexpression of integrin βPS reduces p-MLC enrichment in the NC cortex, whereas its knockdown in SFCs of dumpless1-/- mutants partially rescues NC dumping defect. Our findings identify dumpless1 as a critical effector of ecdysone signaling, bridging somatic-germline communication through the integrin βPS-Rho1-p-MLC axis, revealing a multicellular regulatory mechanism in Drosophila oogenesis.
蜕皮激素信号诱导的dumpless1表达控制果蝇卵发生过程中的乳细胞倾倒。
护理细胞(NC)倾倒是卵母细胞发育的一个重要过程,涉及细胞质从种系来源的NC快速转移到卵母细胞。然而,其监管机制尚不清楚。在这里,我们报道了拉伸卵泡细胞(sfc)中的脱皮激素信号通过dumpless1(一种ZAD-C2H2锌指转录因子)调节果蝇的NC转储。Ecdysone诱导sfc中dumpless1的表达,CRISPR/ cas9介导的敲除dumpless1或其功能域ZAD可抑制NC倾倒。dumpless1的缺失上调了SFC质膜中整合素βPS的表达,同时减少了Rho1信号依赖性磷酸化肌球蛋白轻链(p-MLC)的皮质富集,并破坏了nc中肌动蛋白电缆的组织。sfc特异性整合素βPS的过表达减少了NC皮层中p-MLC的富集,而在dumpless1-/-突变体的sfc中,整合素βPS的下调部分地挽救了NC倾倒缺陷。我们的研究发现,dumpless1是蜕皮激素信号的关键效应者,通过整合素βPS-Rho1-p-MLC轴连接体细胞与种系的通信,揭示了果蝇卵子发生的多细胞调控机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Nature Communications
Nature Communications Biological Science Disciplines-
CiteScore
24.90
自引率
2.40%
发文量
6928
审稿时长
3.7 months
期刊介绍: Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.
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