A lysosome-target fluorescence turn-on probe for exogenous and endogenous HClO detection and its biological applications

IF 5.4 3区化学 Q1 CHEMISTRY, INORGANIC & NUCLEAR

Inorganic Chemistry Communications Pub Date : 2025-09-25 DOI:10.1016/j.inoche.2025.115549

Wen Qin , Ya-Tong Liu , Guiying Hu , Yan Zhao , Chuanbin Fan , Yue-Li Zou , Zhiyong Xing

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Abstract

A new fluorescence probe QW-Lyso conjugated by isophorone and 2-hydroxy-1-naphthaldehyde was synthesized and characterized by ¹H NMR, ¹³C NMR, HRMS and X-ray Crystallography. The probe demonstrated outstanding selectivity and high sensitivity to HClO with an obvious color change and fluorescence turn on response, and the limit of detection was calculated as 85 nM. Furthermore, the possibility mechanism was verified by ¹H NMR titration, HRMS, and DFT calcualtion. Moreover, QW-Lyso was successfully used to image both endogenous and exogenous HClO in living cells, zebrafish, and mice. Colocalization investigation demonstrated that probe QW-Lyso had good lysosomal-targeting and high-contrast imaging abilities, which provided a beneficial tool for the development of HClO fluorescent probe with more efficient application in biological imaging.

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用于外源性和内源性HClO检测的溶酶体靶荧光开启探针及其生物学应用

合成了异佛酮- 2-羟基-1-萘醛偶联荧光探针QW-Lyso，并用1H NMR、13C NMR、HRMS和x射线晶体学对其进行了表征。该探针对HClO具有良好的选择性和高灵敏度，具有明显的颜色变化和荧光开启响应，计算出检测限为85 nM。通过1H NMR滴定、HRMS和DFT计算验证了其可能机理。此外，QW-Lyso还成功地应用于活细胞、斑马鱼和小鼠的内源性和外源性HClO成像。共定位研究表明，QW-Lyso探针具有良好的溶酶体靶向性和高对比度成像能力，为HClO荧光探针的开发提供了有利的工具，更有效地应用于生物成像。

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来源期刊

Inorganic Chemistry Communications 化学-无机化学与核化学

CiteScore

5.50

自引率

7.90%

发文量

1013

审稿时长

53 days

期刊介绍： Launched in January 1998, Inorganic Chemistry Communications is an international journal dedicated to the rapid publication of short communications in the major areas of inorganic, organometallic and supramolecular chemistry. Topics include synthetic and reaction chemistry, kinetics and mechanisms of reactions, bioinorganic chemistry, photochemistry and the use of metal and organometallic compounds in stoichiometric and catalytic synthesis or organic compounds.