Multivariate optimization of selective pressurized liquid extraction for bioactive all-trans fucoxanthin and its isomers from Undaria pinnatifida

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-10-01 DOI:10.1016/j.algal.2025.104336

Paula Garcia-Oliveira , Jesus Simal-Gandara , Lucía Cassani

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Abstract

This study aimed to develop an efficient and sustainable method to recover both all-trans fucoxanthin (FX) and its cis isomers from Undaria pinnatifida (UP). Pressurized liquid extraction (PLE) conditions (time, temperature, and ethanol in ethyl acetate) were optimized using multivariate analysis to maximize FX yield. Temperature notably influenced extraction, with cis and trans isomers showing similar thermal stability. Ethyl acetate showed higher selectivity than ethanol for isolating FX isomers. The optimal PLE conditions were: 115 °C, 100 % ethyl acetate, and 1 min. The recovery of FX isomers was further enhanced by performing three extraction cycles, resulting in a twofold increase in all-trans and cis isomers. The FX-rich extract showed antioxidant capacity, anti-inflammatory properties, and potent anticancer effects against lung carcinoma NCI-H460 and breast adenocarcinoma MCF-7 cell lines. To our knowledge, this is the first systematic evaluation of cis-FX extraction from UP using PLE. Overall, this study provides an innovative, standardized approach for selectively extracting bioactive FX isomers, unlocking their health potential and advancing sustainable valorization of brown algae.

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裙带菜中生物活性全反式岩藻黄素及其异构体的多因素优选

本研究旨在建立一种高效、可持续地从裙带菜（UP）中提取全反式岩藻黄质（FX）及其顺式异构体的方法。利用多变量分析优化了加压液体萃取（PLE）条件（时间、温度和乙酸乙酯乙醇），以最大限度地提高FX收率。温度显著影响萃取，顺式和反式异构体表现出相似的热稳定性。乙酸乙酯对FX异构体的选择性高于乙醇。最佳条件为：115℃，100%乙酸乙酯，1 min。通过三次萃取循环，FX异构体的回收率进一步提高，导致全反式和顺式异构体的回收率增加了一倍。富fx提取物对肺癌NCI-H460和乳腺癌MCF-7细胞具有抗氧化、抗炎和强抗癌作用。据我们所知，这是第一个使用PLE从UP提取cis-FX的系统评估。总的来说，本研究为选择性提取生物活性FX异构体提供了一种创新的、标准化的方法，释放了它们的健康潜力，促进了褐藻的可持续发展。

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来源期刊

Algal Research-Biomass Biofuels and Bioproducts BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

9.40

自引率

7.80%

发文量

332

期刊介绍： Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment