Role of aqueous fenugreek (Trigonella foenum-graecum) extract against DBP-induced renal and adrenal pathologies in male rats

IF 2.2 4区 生物学 Q3 CELL BIOLOGY
Hifsa Shaheen, Sajida Batool, Fatima Iram, Saira Batool, Tahira Almas, Ameer Hamza, Asifa Ijaz, Samar Ambreen, Hafiz Muhammad Uzair
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Abstract

The research was planned to assess the ameliorative effects of fenugreek (FG) against DBP-induced functional and structural changes in the kidney and adrenal glands of male rats. The forty pubertal male rats were distributed (n = 10) into four groups: (i) Control: normal feed and drinking water; (ii) DBP: 250 mg/kg/BW of DBP dissolved in corn oil; (iii) FG: 200 mg/kg /BW of fenugreek aqueous extract; and (iv) DBP + FG: 250 mg/kg/BW of DBP dissolved in corn oil + 200 mg/kg /BW of fenugreek aqueous extract. All doses were administered through oral gavage. Following a 28-day experiment, we used the cervical dislocation technique to euthanize the animals and collect blood samples and organs. The results indicated that DBP causes adverse effects on serum urea, creatinine, and uric acid; malondialdehyde level and catalase activity; average cross-sectional area (ACSA) of glomeruli, Bowman’s capsule, proximal & distal tubules of the kidney, and the mean number of glomerulosa (G), fasciculata (F), reticularis (R), and adrenal medulla (M); average width of adrenal medulla; and ACSA of the adrenal medulla of the adrenal gland. Conversely, concurrent treatment with FG substantially attenuated the kidney and adrenal glands’ toxic effects of DBP. In conclusion, FG, using its antioxidant potential, would be an important phytonutrient against the DBP-intoxicated kidney and adrenal gland.

Abstract Image

胡芦巴水提物对dbp诱导的雄性大鼠肾脏和肾上腺病变的作用。
本研究拟评估胡芦巴(FG)对dbp诱导的雄性大鼠肾脏和肾上腺功能和结构改变的改善作用。将40只青春期雄性大鼠(n = 10)分为4组:(i)对照组:正常喂养和饮水;(ii) DBP:玉米油中溶解DBP 250 mg/kg/BW;(iii) FG:胡芦巴水提物200 mg/kg /BW;(iv) DBP + FG:玉米油溶解DBP 250 mg/kg/BW +胡芦巴水提物200 mg/kg/BW。所有剂量均通过灌胃给药。经过28天的实验,我们采用颈椎脱位技术对动物实施安乐死,并采集血液和器官样本。结果表明,舒张压对血清尿素、肌酐和尿酸有不良影响;丙二醛水平与过氧化氢酶活性;肾小球、鲍曼囊、肾近端和远端小管的平均横截面积(ACSA),肾小球(G)、束状肌(F)、网状肌(R)和肾上腺髓质(M)的平均数目;肾上腺髓质平均宽度;肾上腺髓质的ACSA。相反,与FG同时治疗可显著减弱舒张压对肾脏和肾上腺的毒性作用。综上所述,FG利用其抗氧化潜能,可能是抗dbp中毒肾脏和肾上腺的重要植物营养素。
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来源期刊
Journal of Molecular Histology
Journal of Molecular Histology 生物-细胞生物学
CiteScore
5.90
自引率
0.00%
发文量
68
审稿时长
1 months
期刊介绍: The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.
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