{"title":"Off-line measurement of carbon stable isotopes of trisaccharides, disaccharides, glucose and fructose in honey samples.","authors":"Matheus C Carvalho","doi":"10.1080/10256016.2025.2559196","DOIUrl":null,"url":null,"abstract":"<p><p>Online liquid chromatography (LC) followed by isotope ratio mass spectrometry (IRMS) is a trusted technique to detect honey adulteration based on the stable carbon isotope composition (δ<sup>13</sup>C) of trisaccharides, disaccharides, glucose and fructose. However, LC-IRMS demands specialised analytical setups not commonly found in most isotope labs. Here we investigate the use of off-line LC followed by elemental analysis (EA)-IRMS as an alternative employing more commonly available instruments. Precision and accuracy were excellent for fructose and glucose (error < 0.1 mUr), but less so for disaccharides and trisaccharides, which are similar results to online LC-IRMS. Therefore, offline LC-EA-IRMS can be a viable alternative for the analysis of honey purity employing stable carbon isotopes.</p>","PeriodicalId":14597,"journal":{"name":"Isotopes in Environmental and Health Studies","volume":" ","pages":"1-5"},"PeriodicalIF":1.4000,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Isotopes in Environmental and Health Studies","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1080/10256016.2025.2559196","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, INORGANIC & NUCLEAR","Score":null,"Total":0}
引用次数: 0
Abstract
Online liquid chromatography (LC) followed by isotope ratio mass spectrometry (IRMS) is a trusted technique to detect honey adulteration based on the stable carbon isotope composition (δ13C) of trisaccharides, disaccharides, glucose and fructose. However, LC-IRMS demands specialised analytical setups not commonly found in most isotope labs. Here we investigate the use of off-line LC followed by elemental analysis (EA)-IRMS as an alternative employing more commonly available instruments. Precision and accuracy were excellent for fructose and glucose (error < 0.1 mUr), but less so for disaccharides and trisaccharides, which are similar results to online LC-IRMS. Therefore, offline LC-EA-IRMS can be a viable alternative for the analysis of honey purity employing stable carbon isotopes.
期刊介绍：
Isotopes in Environmental and Health Studies provides a unique platform for stable isotope studies in geological and life sciences, with emphasis on ecology. The international journal publishes original research papers, review articles, short communications, and book reviews relating to the following topics:
-variations in natural isotope abundance (isotope ecology, isotope biochemistry, isotope hydrology, isotope geology)
-stable isotope tracer techniques to follow the fate of certain substances in soil, water, plants, animals and in the human body
-isotope effects and tracer theory linked with mathematical modelling
-isotope measurement methods and equipment with respect to environmental and health research
-diagnostic stable isotope application in medicine and in health studies
-environmental sources of ionizing radiation and its effects on all living matter
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
