Novel terbium-sensitizing peptide substrates for cyclin-dependent kinase 5 (CDK5) and their demonstration in luminescence kinase assays.

IF 3.1 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Jason L Heier, Dylan J Boselli, Laurie L Parker
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引用次数: 0

Abstract

Novel time-resolved terbium luminescence assays were developed for CDK5 and CDK2 by designing synthetic substrates which incorporate phospho-inducible terbium sensitizing motifs with kinase substrate consensus sequences. A substrate designed for CDK5 showed no phosphorylation by CDK2, opening the possibility for CDK5-specific assay development for selective drug discovery.

细胞周期蛋白依赖性激酶5 (CDK5)的新型铽增敏肽底物及其在发光激酶检测中的证明。
通过设计合成底物,将磷诱导的铽敏化基序与激酶底物一致序列结合起来,开发了CDK5和CDK2的新型时间分辨铽发光测定方法。为CDK5设计的底物没有被CDK2磷酸化,这为CDK5特异性检测开发选择性药物提供了可能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
6.10
自引率
0.00%
发文量
128
审稿时长
10 weeks
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