[A Screening Study of GP.Mur Antigen in Blood Donors in Jiangsu Region].

Q4 Medicine

中国实验血液学杂志 Pub Date : 2025-08-01 DOI:10.19746/j.cnki.issn.1009-2137.2025.04.033

Lei Shao, Tai-Xiang Liu, Ling Ma, Fang Zhao, Ruo-Yang Zhang, Hong Lin

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引用次数: 0

Abstract

Objective: To investigate the distribution of GP.Mur antigen in blood donors in Jiangsu Province.

Methods: Genomic DNA was extracted from 1 114 blood donors in Jiangsu region. PCR-SSP was performed to amplify GP.Mur, and gene analysis was conducted by direct sequencing of the PCR products. The frequency of GP.Mur in the blood donor population of Jiangsu region was calculated.

Results: Out of 1 114 randomly selected blood samples, 11 positive bands were detected during amplification. Direct sequencing analysis revealed that among the 11 positive samples, 4 were homozygous for GYP .Mur genotype, 3 were heterozygous for GYP .Mur genotype, and the remaining 4 samples were identified as GYP .HF genotype.

Conclusion: This study analyzed the distribution of the GP.Mur antigen and preliminary obtained the frequency data in the blood donor population in Jiangsu region. Further in-depth research on this blood group is of great importance in guiding clinical blood transfusion practices and ensuring transfusion safety.

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GP筛查研究。江苏地区献血者血清Mur抗原分析[j]。

目的：了解GP的分布情况。江苏省献血者血清Mur抗原分析方法：对江苏地区1114名献血者进行基因组DNA提取。PCR-SSP扩增GP。通过PCR产物的直接测序进行基因分析。GP的频率。计算了江苏地区献血者的死亡率。结果：随机抽取1 114份血样，扩增时检出11条阳性条带。直接测序结果显示，11份阳性样本中有4份为GYP纯合子。其中3个基因型为GYP杂合子。Mur基因型，其余4份鉴定为GYP。高频基因型。结论：本研究分析了GP的分布。初步获得了江苏地区献血者人群中Mur抗原的频率数据。进一步深入研究该血型对指导临床输血实践、保障输血安全具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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