Protoplasting of Aspergillus niger using Trichoderma harzianum culture medium after growth on cell walls of the target fungus

IF 1.9 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-09-09 DOI:10.1016/j.mimet.2025.107252

Juan Pablo Morán Torres, Han A.B. Wösten, Luis G. Lugones

引用次数: 0

Abstract

Protoplast yield from Aspergillus niger can be low when using commercial lytic enzymes from Trichoderma harzianum. This study shows that the culture medium of T. harzianum after growth on A. niger cell walls is much more effective in protoplasting than commercial lysing enzymes.

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黑曲霉在目标真菌细胞壁上生长后用哈茨木霉培养基进行原生质体研究。

当使用哈茨木霉的商业裂解酶时，黑曲霉的原生质体产量可能很低。本研究表明，在黑曲霉细胞壁上生长后的哈兹黑曲霉培养基在原生质体过程中比商业裂解酶更有效。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.