Analysis of proteins in the peripheral and central regions of amphibian oocytes and eggs

Abstract

Proteins in oocytes, meiotically mature eggs and zygotes of Xenopus laevis were examined to determine whether proteins in the peripheral region differ from those in the central region of these cells. We show that different regions contain different amounts of newly synthesized proteins and that during meiotic maturation and fertilization the periphery of the animal hemisphere becomes the site where most newly synthesized proteins are found. Examination of two-dimensional gels indicates that most of these proteins are found in all parts of the egg, but certain proteins demonstrate patterns of distribution which are indicative of (1) polarity, (2) developmental stage, and (3) the position within the hemisphere (central or peripheral). These results suggest that the periphery of oocytes, eggs, and zygotes is a site of greater metabolic activity compared with the central region.