Harnessing conserved G-quadruplexes for fluorescence detection of Mycobacterium tuberculosis

IF 4.2 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Communications Pub Date : 2025-09-09 DOI:10.1039/D5CC04932F

Vasudhar Bhat S. V., Sumon Pratihar, Krithi K. Bhagavath, Mohamed Nabeel Mattath and Thimmaiah Govindaraju

引用次数: 0

Abstract

We report the fluorescence detection of the conserved G-quadruplex (GQ) motif in Mycobacterium tuberculosis (MTB) DNA, ensuring high specificity with minimal interference from human and non-MTB DNA sequences. The duplex-to-GQ reliable conformational polymorphism (GQ-RCP) enables its application in amplification-compatible MTB detection, offering a platform for precise diagnostic assays.

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利用保守g -四重复合物荧光检测结核分枝杆菌

我们报道了在结核分枝杆菌（MTB） DNA中保守的g -四重体（GQ）基序的荧光检测，确保了高特异性，并且受到人类和非结核分枝杆菌DNA序列的干扰最小。双工- gq可靠构象多态性（GQ-RCP）使其能够应用于扩增兼容的MTB检测，为精确诊断分析提供了一个平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemical Communications 化学-化学综合

CiteScore

8.60

自引率

4.10%

发文量

2705

审稿时长

1.4 months

期刊介绍： ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.