First record of Rickettsia bellii in Amblyomma varium and Amblyomma goeldii from the Eastern Amazon.

IF 1.2 4区农林科学 Q2 Veterinary

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2025-09-01 eCollection Date: 2025-01-01 DOI:10.1590/S1984-29612025043

Izabela Mesquita Araújo, Kelly Karoline Gomes do Nascimento, Mateus Borges Silva, Isis Abel, Ana Cristina Mendes-Oliveira, Bruna de Azevedo Baêta, Claudia Bezerra da Silva, Matheus Dias Cordeiro

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Abstract

This study investigated the presence of Rickettsia spp., Ehrlichia spp., Anaplasma spp., and Borrelia spp. DNA in questing ticks collected from a forest fragment under constant anthropogenic pressure in the state of Pará, Brazil. The fragment was divided into three zones: interior, edge, and surrounding matrix. Ticks were collected in all zones using flannel drag sampling, flannel leggings, and CO2-baited traps. Nymphs and adults were morphologically identified using taxonomic keys, while larvae were subjected to molecular identification. DNA extracted from the ticks was tested for the target pathogens using polymerase chain reaction (PCR). In total, 561 questing ticks (525 larvae, 29 nymphs, and 7 adults) were collected, representing eight species: Amblyomma cajennense sensu stricto, Amblyomma calcaratum, Amblyomma geayi, Amblyomma goeldii, Amblyomma humerale, Amblyomma naponense, Amblyomma pacae, and Amblyomma varium. Rickettsia bellii was detected in one A. varium larva and one A. goeldii larva. All samples tested negative for Ehrlichia spp., Anaplasma spp., and Borrelia spp. This is the first report of R. bellii in A. varium and A. goeldii in Brazil.

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首次在亚马逊河东部的各种钝眼虫和goeldii钝眼虫中发现贝氏立克次体。

本研究调查了在巴西帕尔州持续的人为压力下从森林碎片中收集的蜱虫中立克次体、埃利希体、无形体和伯氏疏螺旋体的DNA存在。碎片被划分为三个区域：内部、边缘和周围矩阵。使用法兰绒拖拽取样、法兰绒绑腿和二氧化碳诱捕器在所有区域收集蜱虫。用分类键对若虫和成虫进行形态鉴定，对幼虫进行分子鉴定。采用聚合酶链式反应（PCR）检测蜱体DNA的目标病原体。共捕获吸血蜱561只，其中幼虫525只，稚虫29只，成虫7只，分别代表8个蜱种：狭义长尾无蛉、足形无蛉、格尾无蛉、goeldi无蛉、人类无蛉、naponense无蛉、pacae无蛉、varium无蛉。分别检出1只变异姬鼠和1只戈伊地姬鼠的贝氏立克次体。所有样本的埃利希氏体、无形体和伯氏疏螺旋体检测均为阴性。这是巴西首次报告在阿凡达和戈尔地阿凡达中发现贝氏疏螺旋体。

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来源期刊

Revista Brasileira De Parasitologia Veterinaria PARASITOLOGY-VETERINARY SCIENCES

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： La revista es un órgano de difusión del Colegio Brasileño de Parasitología Veterinaria, con una especificidad dentro de esa área, la difusión de los resultados de la investigación brasileña en las áreas de Helmintología, Protozoología, Entomología y agentes transmitidos por artrópodos, relacionados con la salud animal.