Molecular Impacts of Occupational Exposure to Waste Anesthetic Gases

IF 2.3 4区医学 Q3 ENVIRONMENTAL SCIENCES

Environmental and Molecular Mutagenesis Pub Date : 2025-08-25 DOI:10.1002/em.70029

Mariane A. P. Silva, Mónica Cappetta, Tony F. Grassi, Leandro G. Braz, Mariana G. Braz

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Abstract

Millions of healthcare professionals are occupationally exposed to waste anesthetic gases (WAGs) worldwide; however, their effect on DNA methylation is unknown. Thus, for the first time, global DNA methylation analysis was performed in addition to the comet assay and gene expression analysis in this case–control study to reveal possible molecular alterations associated with WAG exposure. A total of 86 participants were included (43 healthcare professionals exposed to WAGs and 43 matched-controls). Mononuclear cells were isolated for the comet assay, and DNA was extracted from whole blood for global DNA methylation analysis. RNA was isolated for the gene expression of NRF2, SIRT1, and HO1. Increased DNA damage was observed in the exposed group (p = 0.02). No differences in global DNA methylation or HO1 expression were observed (p > 0.05), but SIRT1 (p = 0.01) and NRF2 (p = 0.02) upregulation were observed in the exposed group. These findings highlight the molecular effects of WAG exposure.

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职业接触废麻醉气体的分子影响。

全世界数以百万计的卫生保健专业人员在职业上暴露于废麻醉气体（wag）；然而，它们对DNA甲基化的影响尚不清楚。因此，在本病例对照研究中，除了彗星分析和基因表达分析外，还首次进行了全球DNA甲基化分析，以揭示与WAG暴露相关的可能的分子改变。总共包括86名参与者（43名暴露于wag的医疗保健专业人员和43名匹配的对照组）。分离单个核细胞用于彗星试验，从全血中提取DNA用于全局DNA甲基化分析。分离RNA用于NRF2、SIRT1和HO1的基因表达。暴露组DNA损伤加重（p = 0.02）。暴露组整体DNA甲基化和HO1表达无差异（p > 0.05），但SIRT1 （p = 0.01）和NRF2 （p = 0.02）上调。这些发现突出了WAG暴露的分子效应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Environmental and Molecular Mutagenesis 环境科学-毒理学

CiteScore

5.40

自引率

10.70%

发文量

审稿时长

12-24 weeks

期刊介绍： Environmental and Molecular Mutagenesis publishes original research manuscripts, reviews and commentaries on topics related to six general areas, with an emphasis on subject matter most suited for the readership of EMM as outlined below. The journal is intended for investigators in fields such as molecular biology, biochemistry, microbiology, genetics and epigenetics, genomics and epigenomics, cancer research, neurobiology, heritable mutation, radiation biology, toxicology, and molecular & environmental epidemiology.