Exploring the potential bioactive compounds group and mechanism of Ci Bai Capsule in treating leukopenia: a combined approach of network pharmacology and transcriptome evidences.

IF 5.7 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Chinese Medicine Pub Date : 2025-09-01 DOI:10.1186/s13020-025-01197-9

Dingfan Zhang, Congshu Huang, Lei Zhou, Boyang Wang, Wei Zhou, Tiantian Xia, Pan Shen, Shao Li, Yue Gao

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Abstract

Background: Radiation-induced leukopenia caused by low-dose exposure is frequently associated with Traditional Chinese Medicine (TCM) syndromes like "blood deficiency" and "fatigue syndrome". Ci Bai Capsule (CB) has been reported to enhance white blood cell levels; however, its mechanisms and bioactive compounds remain unclear.

Aim: This study aimed to identify the bioactive compounds group of CB and elucidate its potential mechanisms in radiation-induced leukopenia.

Methods: Syndrome-related data were gathered from SYMMAP and CTD database. CB's target profile is predicted by DrugCIPHER. Network pharmacology approaches were employed to identify active compounds and related pathways. Experimental validation was conducted through flow cytometry, RNA-sequencing both ex vivo and in vivo models. RT-qPCR and Western blot were performed for quantitative validation of key targets.

Results: A total of 22 pathways related to cellular processes, immune responses, and signal transduction were identified. Five key bioactive compounds (kaempferol-3-glucorhamnoside, syringin, schisandrin, 3-hydroxytyrosol 3-O-glucoside and salidroside) were found to significantly modulate syndrome-related pathways. Optimal dosing of this compound combination enhanced leukocyte counts and splenic immune cell proliferation in irradiated mice. Transcriptomic analysis revealed that the compounds exert regulatory effects on PP1A, RB, CDK4/6, CDK2, and CDK1, thereby modulating downstream immune and hematopoietic markers such as MNDA, BST2, and HSPA1A.

Conclusion: Our findings suggest that CB mitigates radiation-induced leukopenia by enhancing immune and hematopoietic recovery, offering a promising therapeutic approach for managing radiation-related hematological disorders.

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网络药理学和转录组学证据相结合的方法探讨慈白胶囊治疗白细胞减少症的潜在活性化合物群及其作用机制。

背景：低剂量照射引起的辐射性白细胞减少常与“血虚”、“疲劳综合征”等中医证候有关。慈白胶囊（CB）有提高白细胞水平的报道；然而，其作用机制和生物活性成分尚不清楚。目的：本研究旨在鉴定白炭黑的生物活性化合物群，并阐明其在辐射致白细胞减少中的潜在机制。方法：从SYMMAP和CTD数据库中收集证候相关数据。用DrugCIPHER预测CB的靶谱。网络药理学方法用于鉴定活性化合物和相关途径。通过流式细胞术、体外和体内模型的rna测序进行实验验证。采用RT-qPCR和Western blot对关键靶点进行定量验证。结果：共鉴定出22条与细胞过程、免疫反应和信号转导相关的通路。发现5种关键生物活性化合物（山奈酚-3-葡萄糖苷、紫丁香苷、五味子苷、3-羟基酪醇3- o -葡萄糖苷和红景天苷）可显著调节综合征相关通路。该化合物组合的最佳剂量增强了受照射小鼠的白细胞计数和脾免疫细胞增殖。转录组学分析显示，这些化合物对PP1A、RB、CDK4/6、CDK2和CDK1具有调节作用，从而调节下游免疫和造血标志物，如MNDA、BST2和HSPA1A。结论：我们的研究结果表明，CB通过增强免疫和造血恢复来减轻辐射引起的白细胞减少，为治疗辐射相关血液疾病提供了一种有希望的治疗方法。

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来源期刊

Chinese Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-PHARMACOLOGY & PHARMACY

CiteScore

7.90

自引率

4.10%

发文量

133

审稿时长

31 weeks

期刊介绍： Chinese Medicine is an open access, online journal publishing evidence-based, scientifically justified, and ethical research into all aspects of Chinese medicine. Areas of interest include recent advances in herbal medicine, clinical nutrition, clinical diagnosis, acupuncture, pharmaceutics, biomedical sciences, epidemiology, education, informatics, sociology, and psychology that are relevant and significant to Chinese medicine. Examples of research approaches include biomedical experimentation, high-throughput technology, clinical trials, systematic reviews, meta-analysis, sampled surveys, simulation, data curation, statistics, omics, translational medicine, and integrative methodologies. Chinese Medicine is a credible channel to communicate unbiased scientific data, information, and knowledge in Chinese medicine among researchers, clinicians, academics, and students in Chinese medicine and other scientific disciplines of medicine.