Prevalence and zoonotic potential of Enterocytozoon bieneusi in dogs and cats in Guangdong, China

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-08-21 DOI:10.1016/j.vprsr.2025.101334

Na Li, Xiangming Liu, Xiaoyu Dan, Jiayu Li, Rui Xu, Lihua Xiao, Yaoyu Feng, Yaqiong Guo

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Abstract

Dogs and cats are important reservoirs for the zoonotic pathogen Enterocytozoon bieneusi. In this study, fecal samples from 682 dogs and 424 cats in Guangdong Province, China, were examined for E. bieneusi by PCR targeting the internal transcribed spacer (ITS) of the rRNA gene. The overall infection rate was 31.5 % in dogs and 3.5 % in cats. Significantly higher infection rates were observed in dogs >6 months (31.0 %, 137/442, χ² = 33.168, P = 0.000), female dogs (35.0 %, 114/326, χ² = 22.669, P = 0.000), purebred dogs (29.7 %, 140/471, χ² = 24.464, P = 0.000), dogs from the research center and shelters (52.3 %, 116/222, P = 0.000; 50.7 %, 71/140, P = 0.000). The genotypes detected in dogs included canine-adapted PtEb IX (n = 212) and WW8 (n = 2), and zoonotic Type IV (n = 1). In cats, Type IV was dominant (n = 12), followed by D (n = 2) and PtEb IX (n = 1). The genetic diversity in Type IV and D samples were further assessed by multilocus sequence typing at four microsatellite/minisatellite loci. Twelve multilocus genotypes (MLGs) were identified, suggesting inconsistent genotyping results among the loci. Population genetic analysis of the MLG data generated in this study and those from other hosts in previous studies revealed three distinct subpopulations. Subpopulation 1 and Subpopulation 3 contained E. bieneusi from monkeys and fur animals, respectively. In contrast, Subpopulation 2 contained E. bieneusi from humans and cats. This suggests that E. bieneusi in cats and in humans is genetically related and has the potential for zoonotic transmission.

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广东犬、猫双胞虫患病率及人畜共患潜力

狗和猫是人畜共患病原体双胞虫的重要宿主。本研究对广东省682只狗和424只猫的粪便样本进行了针对rRNA基因内转录间隔段（ITS）的PCR检测。狗和猫的总感染率分别为31.5%和3.5%。6月龄犬（31.0%,137/442,χ2 = 33.168, P = 0.000）、母犬（35.0%,114/326,χ2 = 22.669, P = 0.000）、纯种犬（29.7%,140/471,χ2 = 24.464, P = 0.000）、研究中心和收容所的犬（52.3%,116/222,P = 0.000; 50.7%, 71/140, P = 0.000）的感染率均显著高于母犬（31.0%,137/442,χ2 = 33.168, P = 0.000）。在犬中检测到的基因型包括犬适应型PtEb IX （n = 212）和WW8 (n = 2)，以及人畜共患型IV （n = 1）。在猫中，IV型占主导地位（n = 12），其次是D型（n = 2）和PtEb IX型（n = 1）。通过4个微卫星/小卫星位点的多位点序列分型进一步评估了IV型和D型样品的遗传多样性。共鉴定出12个多位点基因型（mlg），表明基因座之间的基因分型结果不一致。对本研究中产生的MLG数据和先前研究中来自其他宿主的数据进行种群遗传分析，揭示了三个不同的亚种群。亚种群1和亚种群3分别含有来自猴子和毛皮动物的别氏伊蚊。相比之下，亚群2含有来自人类和猫的比氏伊蚊。这表明，猫和人类中的布氏伊布氏体具有遗传相关性，并具有人畜共患传播的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).