Recombinant Bacillus subtilis Displaying DHAV-1 VP1 Protein as a Dual-Function Probiotic: Evaluation of Immunological Efficacy, Growth Performance, Antioxidant Capacity, and Intestinal Health in Cherry Valley Ducks.

IF 4.4 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Probiotics and Antimicrobial Proteins Pub Date : 2025-08-15 DOI:10.1007/s12602-025-10720-1

Bin Chen, Yicen Tang, Lihui Huang, Zhenhua Wang, Jianzhen Li, Mengwei Zhang, Yang Yang, Dongmei Zhang, Bo Jing, Yan Zeng, Xueqin Ni, Jinlong Yang, Kangcheng Pan

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引用次数: 0

Abstract

Duck hepatitis A virus type 1 (DHAV-1) demonstrates extremely high lethality in 3-week-old ducklings, while causing subclinical infections in adult ducks with latent pathogenicity. Our previous research developed a vaccine-functional probiotic preparation targeting DHAV-1-recombinant Bacillus subtilis RV. In the present study, we conducted a 28-day oral immunization trial in Cherry Valley ducks to evaluate the immunoprotective effects and probiotic functions of this engineered strain. Results showed that B. subtilis RV induced humoral and mucosal immune responses in ducklings, producing high levels of specific IgG and intestinal secretory IgA, demonstrating immune-enhancing capabilities. During DHAV-1 challenge tests, it significantly reduced viral loads in various organ tissues. Additionally, B. subtilis RV improved the growth performance and antioxidant capacity of ducklings. Furthermore, this engineered probiotic enhanced intestinal health by improving intestinal structure, increasing tight junction protein expression, and modulating gut microbiota composition. This study evaluates a novel vaccine-functional probiotic preparation, providing a new strategy for DHAV-1 prevention and control.

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显示DHAV-1 VP1蛋白的重组枯草芽孢杆菌作为双功能益生菌：樱桃谷鸭的免疫功效、生长性能、抗氧化能力和肠道健康评价

鸭甲型肝炎病毒1型（DHAV-1）在3周龄雏鸭中表现出极高的致死率，而在成年鸭中引起亚临床感染，具有潜伏致病性。我们之前的研究开发了一种针对dhav -1重组枯草芽孢杆菌RV的疫苗功能益生菌制剂。在本研究中，我们对樱桃谷鸭进行了为期28天的口服免疫试验，以评估该工程菌株的免疫保护作用和益生菌功能。结果表明，枯草芽孢杆菌RV可诱导雏鸭的体液和粘膜免疫反应，产生高水平的特异性IgG和肠道分泌IgA，具有免疫增强能力。在DHAV-1攻击试验中，它显著降低了各种器官组织中的病毒载量。此外，枯草芽孢杆菌RV还能提高雏鸭的生长性能和抗氧化能力。此外，该工程益生菌通过改善肠道结构、增加紧密连接蛋白表达和调节肠道菌群组成来改善肠道健康。本研究评价了一种新的疫苗功能益生菌制剂，为预防和控制DHAV-1提供了新的策略。

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来源期刊

Probiotics and Antimicrobial Proteins BIOTECHNOLOGY & APPLIED MICROBIOLOGYMICROB-MICROBIOLOGY

CiteScore

11.30

自引率

6.10%

发文量

140

期刊介绍： Probiotics and Antimicrobial Proteins publishes reviews, original articles, letters and short notes and technical/methodological communications aimed at advancing fundamental knowledge and exploration of the applications of probiotics, natural antimicrobial proteins and their derivatives in biomedical, agricultural, veterinary, food, and cosmetic products. The Journal welcomes fundamental research articles and reports on applications of these microorganisms and substances, and encourages structural studies and studies that correlate the structure and functional properties of antimicrobial proteins.