Comparison of Xpert® MTB/RIF and Xpert® MTB/RIF Ultra in pediatric pulmonary tuberculosis diagnosis.

IF 1.1 4区 医学 Q2 PEDIATRICS
Shruti Kaushal, Sunil Kumar Dhatwalia, Rakesh Yadav, Sumedha Sharma, Pankaj C Vaidya, Joseph L Mathew, Sunil Sethi
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引用次数: 0

Abstract

The World Health Organization (WHO) recommends Xpert® MTB/RIF (Xpert) and its advanced version, Xpert® MTB/RIF Ultra (Xpert Ultra), as first-line diagnostic tests for detecting pulmonary tuberculosis (PTB) and rifampicin resistance in children suspected of having the disease. Respiratory specimens (gastric lavage/bronchoalveolar lavage/sputum/endotracheal aspirate) obtained from 116 children with presumptive PTB were simultaneously processed using liquid medium culture, Xpert assay, and Xpert Ultra assay. Among the specimens from 116 children, six were excluded due to culture contamination (n = 5) or error in Xpert Ultra results (n = 1). Among the remaining 110 specimens, 20 were positive by liquid culture. The former and latter, of the two comparator tests gave a sensitivity of 90% and 95%, respectively. The respective specificity was 93.3% and 88.9%. Xpert Ultra showed a statistically significant slightly higher sensitivity than Xpert. Xpert Ultra showed slightly higher sensitivity than Xpert, with a minimal loss in specificity, partly due to the inclusion of trace results, which help detect paucibacillary cases.

Xpert®MTB/RIF与Xpert®MTB/RIF Ultra在儿童肺结核诊断中的比较
世界卫生组织(WHO)推荐Xpert®MTB/RIF (Xpert)及其高级版本Xpert®MTB/RIF Ultra (Xpert Ultra)作为一线诊断测试,用于检测疑似患有结核病的儿童的肺结核(PTB)和利福平耐药性。116例疑似肺结核患儿的呼吸道标本(洗胃/支气管肺泡灌洗/痰液/气管内吸入)同时采用液体培养基培养、Xpert法和Xpert Ultra法进行处理。116例儿童标本中,6例因培养污染(n = 5)或Xpert Ultra结果错误(n = 1)而被排除。其余110份标本中,液体培养阳性20份。两种比较试验中前者和后者的灵敏度分别为90%和95%。特异性分别为93.3%和88.9%。Xpert Ultra的灵敏度略高于Xpert。Xpert Ultra的灵敏度略高于Xpert,特异性损失最小,部分原因是包含痕量结果,有助于检测少杆菌病例。
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来源期刊
Journal of Tropical Pediatrics
Journal of Tropical Pediatrics 医学-热带医学
CiteScore
4.00
自引率
0.00%
发文量
97
审稿时长
6-12 weeks
期刊介绍: The Journal of Tropical Pediatrics provides a link between theory and practice in the field. Papers report key results of clinical and community research, and considerations of programme development. More general descriptive pieces are included when they have application to work preceeding elsewhere. The journal also presents review articles, book reviews and, occasionally, short monographs and selections of important papers delivered at relevant conferences.
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