Activity-Based Fluorescent Probes for Reactive Sulfur Species

IF 17.7 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2025-08-12 DOI:10.1021/acs.accounts.5c00483

Ming Xian*, Yuqing Wang, Conrad Du and Meg Shieh,

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引用次数: 0

Abstract

Reactive sulfur species (RSS), such as hydrogen sulfide (H₂S), hydrogen per/polysulfide (H₂S_n, n > 1), hydropersulfides (RSSH), and polysulfides (RS_nR, n > 2), are believed to play regulatory roles in redox biology. However, their exact mechanisms of action still need to be clarified. The instability of various RSS under physiological environments and their highly reactive natures pose unique challenges to the research on these species. Considering these challenges, fluorescent probes that can rapidly and specifically detect each individual RSS in biological settings are critical tools for RSS research. This Account follows our laboratory’s development of such probes.

We began by exploring the specific reactions of H₂S that could distinguish H₂S from other RSS. Based on the reactions, we developed several series of H₂S probes. We then investigated the reactions of other RSS, including H₂S_n, sulfane sulfur, HSNO, etc., and developed the corresponding probes. Our research on these probes also inspired us to develop other related chemical tools, including H₂S scavengers and a general structural template for xanthene-based near-infrared dyes. This Account summarizes our work in this field and systematically explains how each probe/chemical tool was designed and evaluated. This Account covers the following key points: (1) the rational chemical design of each probe template; (2) the evaluation and mechanistic insights for each probe template; and (3) the properties and applications of the probes/chemical tools.

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活性型硫类荧光探针研究。

活性硫物质（RSS），如硫化氢（H2S）、氢/多硫化氢（H2Sn, n > 1）、氢过硫化物（RSSH）和多硫化物（RSnR, n > 2），被认为在氧化还原生物学中起着调节作用。然而，它们的确切作用机制仍然需要澄清。各种RSS在生理环境下的不稳定性和高反应性给这些物种的研究带来了独特的挑战。考虑到这些挑战，荧光探针可以快速和特异性地检测生物环境中的每个RSS个体，是RSS研究的关键工具。本帐户跟踪我们实验室对这种探针的开发。我们首先探索H2S的特定反应，以区分H2S和其他RSS。基于这些反应，我们开发了几个系列的H2S探针。然后，我们研究了其他RSS的反应，包括H2Sn、亚砜硫、HSNO等，并开发了相应的探针。我们对这些探针的研究也启发了我们开发其他相关的化学工具，包括H2S清除剂和基于杂蒽的近红外染料的通用结构模板。本帐户总结了我们在这一领域的工作，并系统地解释了每个探针/化学工具是如何设计和评估的。本课题主要包括以下几个重点：(1)各探针模板的合理化学设计；(2)对每个探针模板的评价和机制见解；(3)探针/化学工具的性质和应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.